Screening for compounds with bioaccumulation potential in breast milk using their retention behavior in two-dimensional gas chromatography

• Published in: Environment international 2024-08, Vol.190, p.108911, Article 108911
• Main Authors: Zhang, Yingxin, Gao, Lirong, Ai, Qiaofeng, Liu, Yang, Qiao, Lin, Cheng, Xin, Li, Jingguang, Zhang, Lei, Lyu, Bing, Zheng, Minghui, Wu, Yongning
• Format: Article
• Language: English
• Subjects: Bioaccumulation potential; Breast milk; China; Chromatography, Gas; Environmental Monitoring - methods; Environmental Pollutants - analysis; Female; GC×GC; Health risk; Humans; Milk, Human - chemistry; Partitioning properties; Phthalic Acids - analysis; Plasticizers - analysis; Prioritization
• ISSN: 0160-4120; 1873-6750; 1873-6750
• DOI: 10.1016/j.envint.2024.108911

[Display omitted] •A method for screening compounds with bioaccumulation potential was developed.•The method decreases the number of compounds needing structural identification.•62 compounds were identified in breast milk, with 27 compounds being found for the first time.•Priority ranking indicated that 13 compounds pose stronger risks. Discovery of emerging pollutants in breast milk will be helpful for understanding the hazards to human health. However, it is difficult to identify key compounds among thousands present in complex samples. In this study, a method for screening compounds with bioaccumulation potential was developed. The method can decrease the number of compounds needing structural identification because the partitioning properties of bioaccumulative compounds can be mapped onto GC×GC chromatograms through their retention behaviors. Twenty pooled samples from seven provinces in China were analyzed. 1,286 compounds with bioaccumulation potential were selected from over 3,000 compounds. Sixty-two compounds, including aromatic compounds, phthalates, and phenolics etc., were identified with a high level of confidence and then quantified. Among them, twenty-seven compounds were found for the first time in breast milk. Three phthalate plasticizers and two phenolic antioxidants were found in significantly higher concentrations than other compounds. A toxicological priority index approach was applied to prioritize the compounds considering their concentrations, detection frequencies and eight toxic effects. The prioritization indicated that 13 compounds, including bis(2-ethylhexyl) phthalate, dibutyl phthalate, 1,3-di-tert-butylbenzene, phenanthrene, 2,6-di-tert-butyl-1,4-benzoquinone, 2,4-di-tert-butylphenol, and others, showed higher health risks. Meanwhile, some compounds with high risk for a particular toxic effect, such as benzothiazole and geranylacetone, were still noteworthy. This study is important for assessing the risks of human exposure to organic compounds.