Effects of porcine reproductive and respiratory syndrome virus (PRRSV) on thyroid hormone metabolism in the late gestation fetus

• Published in: Veterinary research (Paris) 2022-09, Vol.53 (1), p.1-74, Article 74
• Main Authors: Ison, Erin K, Hopf-Jannasch, Amber S, Harding, John C. S, Alex Pasternak, J
• Format: Article
• Language: English
• Subjects: Analysis; Endometrium; Enzymes; Feces; fetal; Fetuses; Gene expression; Hogs; Hormones; host–pathogen interaction; hypothyroidism; Infections; Iodine; Liquid chromatography; Liver; Mass spectrometry; maternal; Metabolism; Metabolites; Physiological aspects; Placenta; Porcine reproductive and respiratory syndrome; Pregnancy; PRRS; Thymus gland; Thyroid gland; thyroid hormone; Thyroid hormones; Viral infections
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-022-01092-3

Porcine reproductive and respiratory syndrome virus (PRRSV) in late gestation causes a profound suppression of circulating maternal and fetal thyroid hormone during a critical window of development. To understand this relationship, we evaluated thyroid hormone metabolism at the maternal–fetal interface and within fetal tissues, along with hormone metabolite levels in serum. Fetuses were classified using an established model based on viral load in serum and thymus, and preservation status, including uninfected (UNIF), high-viral load viable (HV-VIA), and high-viral load meconium-stained (HV-MEC), with additional controls from sham-inoculated gilts (CON). Expression of three iodothyronine deiodinases, five sulfotransferases, sulfatase, and two solute carriers known to transport thyroid hormone were evaluated in maternal endometrium and fetal placenta, liver, and kidney. Serum thyroxin (T4), reverse triiodothyronine (rT3), and diiodothyronine (T2) were evaluated via liquid chromatography tandem mass spectrometry. Significant changes in gene expression were observed in all four tissues, with the liver being the most severely impacted. We observed local and fetal specific regulation of maternal tissues through significant upregulation of DIO2 and DIO3 expression in the endometrium corresponding to infected but viable fetuses relative to uninfected and control fetuses. Expression levels of DIO2 and DIO3 were significantly higher in the resilient (HV-VIA) fetuses relative to the susceptible (HV-MEC) fetuses. A substantial decrease in serum T4 was confirmed, with no corresponding increase in rT3 or T2. Collectively, these results show that thyroid hormone metabolism is altered at the maternal–fetal interface and within the PRRSV infected fetus and is associated with fetal viability.