L, D-Polydeoxyribonucleotides to provide an essential inhibitory effect on DNA polymerase β of human myeloid leukemia HL60 cells

The inhibitory effect of D and L-polynucleotides of a given length (40-50n) on the catalytic activity of DNA polymerase β isolated from chromatin cells of acute myeloid leukemia HL-60 was evaluated. The synthesized L enantiomer was found to have a higher inhibitory activity than the synthesized and...

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Bibliographic Details
Published in:Biochemistry and biophysics reports 2020-12, Vol.24, p.100835-100835, Article 100835
Main Authors: Stovbun, S.V., Vedenkin, A.S., Bukhvostov, A.A., Koroleva, L.S., Silnikov, V.N., Kuznetsov, D.A.
Format: Article
Language:English
Subjects:
DNA Polymerase β
DNA Repair
HL-60
l-thymidine
Leukemia
Polydeoxyribonucleotides
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Summary:The inhibitory effect of D and L-polynucleotides of a given length (40-50n) on the catalytic activity of DNA polymerase β isolated from chromatin cells of acute myeloid leukemia HL-60 was evaluated. The synthesized L enantiomer was found to have a higher inhibitory activity than the synthesized and isolated D enantiomers of polynucleotides. The work also proposes a biophysical model that describes this effect. •The inhibitory activity of L, D-polydeoxyribonucleotides of various compositions was evaluated.•Inhibition of DNA polymerase β is due to a nonspecific interaction between the enzyme and the substrate.•L-polynucleotide exhibits the highest inhibiting activity, compared to the D-enantiomers.
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2020.100835