Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study

The aim of this study was to provide a beneficial treatment effect of mesenchymal stem cell products derived from the canine amniotic membrane (AM-MSC) on the complicated wound healing process in dogs. AM-MSCs were characterized in terms of morphology, phenotypic profile, and multilineage differenti...

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Published in:International journal of molecular sciences 2023-05, Vol.24 (9), p.8214
Main Authors: Humenik, Filip, Maloveská, Marcela, Hudáková, Nikola, Petroušková, Patrícia, Šufliarska, Zuzana, Horňáková, Ľubica, Valenčáková, Alexandra, Kožár, Martin, Šišková, Barbora, Mudroňová, Dagmar, Bartkovský, Martin, Čížková, Daša
Format: Article
Language:English
Subjects:
Adipocytes
Amnion
Amniotic membrane
Angiogenesis
Animals
Antibiotics
Blood vessels
Care and treatment
Cell culture
Cell Proliferation
conditioned media
Culture Media, Conditioned - metabolism
Culture Media, Conditioned - pharmacology
Cytokines
Dogs
fibroblast
Fibroblast growth factor 2
Fibroblast growth factors
Fibroblasts
Growth factors
In vivo methods and tests
mesenchymal stem cell
Mesenchymal stem cells
Mesenchymal Stem Cells - metabolism
Nonsteroidal anti-inflammatory drugs
Skin
Stem cell research
Stem cells
Tissue engineering
Wound Healing
Wounds and injuries
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Summary:The aim of this study was to provide a beneficial treatment effect of mesenchymal stem cell products derived from the canine amniotic membrane (AM-MSC) on the complicated wound healing process in dogs. AM-MSCs were characterized in terms of morphology, phenotypic profile, and multilineage differentiation potential. The in vitro study of the effect of canine amniotic mesenchymal stem cell conditioned media (AMMSC-CM) on a primary skin fibroblast cell culture scratch assay showed a decrease in the measured scratch area of about 66.39% against the negative control (Dulbecco's Modified Eagle's Medium-32.55%) and the positive control (Dulbecco's Modified Eagle's Medium supplemented with FGF2, N2, B27, and EGF-82.077%) after 72 h treatment. In the experimental study, seven dogs with complicated nonhealing wounds were treated with a combination of antibiotics, NSAIDs, and local AMMSC-CM application. After 15 days of therapy, we observed a 98.47% reduction in the wound surface area as opposed to 57.135% in the control group treated by conventional therapy based on debridement of necrotic tissue, antibiotic therapy, pain management, and change of wound dressing.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24098214