Loading…

Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds

Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of medical and biological research 2012-11, Vol.45 (11), p.1002-1010
Main Authors: Fernandes, K V, Deus-de-Oliveira, N, Godoy, M G, Guimarães, Z A S, Nascimento, V V, Melo, E J T de, Freire, D M G, Dansa-Petretski, M, Machado, O L T
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383
cites cdi_FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383
container_end_page 1010
container_issue 11
container_start_page 1002
container_title Brazilian journal of medical and biological research
container_volume 45
creator Fernandes, K V
Deus-de-Oliveira, N
Godoy, M G
Guimarães, Z A S
Nascimento, V V
Melo, E J T de
Freire, D M G
Dansa-Petretski, M
Machado, O L T
description Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 x 10(5) cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH)2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.
doi_str_mv 10.1590/S0100-879X2012007500132
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_0d4c2e99e693440abddb94132b7f1d0e</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S0100_879X2012001100002</scielo_id><doaj_id>oai_doaj_org_article_0d4c2e99e693440abddb94132b7f1d0e</doaj_id><sourcerecordid>1458530022</sourcerecordid><originalsourceid>FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383</originalsourceid><addsrcrecordid>eNplUsFu1DAQjRCILoVfgBy5pIxjexNfkFBVoFIlDgWpN8t2Jlsvjr3YTqF_j7dZFipOtsfz3rx5M1X1hsAZ4QLeXQMBaPpO3LRAWoCOAxDaPqlWhBHWMEpunlarY9JJ9SKlLUDLgZHn1UnbCkIoY6tqurbT7LLyGOZUK-cwbtDX1iuT7Z3KNvha-aEeMIdfdrRmCYWxNirlEGuNypf7d6z1fZ0jqjyhz_VPm29L2Bk7T7UJ0y7Mfkgvq2ejcglfHc7T6tvHi6_nn5urL58uzz9cNYZznps16whdCySt7gkgb7XiqvSyHvoOtGICNHYawYyMma7vRuAClQFOsSNIe3paXS68Q1BbuYt2UvFeBmXlQyDEjVQxW-NQwsBMi0LgWhRDQOlh0IIVL3U3kgGwcJ0tXMlYdEFuwxx9ES8fZiD_zoCUZ7G4AN4vgN2sJxxMsSMq90jF4x9vb-Um3Enac0b4Xv3bA0EMP2ZMWU42GXRumZIkjPecllL7Wt2SamJIKeJ4LENA7jflP5WHTSnI1_-qPOL-rAb9DUoLuag</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1458530022</pqid></control><display><type>article</type><title>Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds</title><source>SciELO Brazil</source><source>IngentaConnect Journals</source><creator>Fernandes, K V ; Deus-de-Oliveira, N ; Godoy, M G ; Guimarães, Z A S ; Nascimento, V V ; Melo, E J T de ; Freire, D M G ; Dansa-Petretski, M ; Machado, O L T</creator><creatorcontrib>Fernandes, K V ; Deus-de-Oliveira, N ; Godoy, M G ; Guimarães, Z A S ; Nascimento, V V ; Melo, E J T de ; Freire, D M G ; Dansa-Petretski, M ; Machado, O L T</creatorcontrib><description>Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 x 10(5) cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH)2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.</description><identifier>ISSN: 0100-879X</identifier><identifier>ISSN: 1414-431X</identifier><identifier>EISSN: 1414-431X</identifier><identifier>EISSN: 0100-879X</identifier><identifier>DOI: 10.1590/S0100-879X2012007500132</identifier><identifier>PMID: 22911344</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Medicina Tropical</publisher><subject>2S albumin ; 2S Albumins, Plant - isolation &amp; purification ; 2S Albumins, Plant - toxicity ; Allergens - drug effects ; Allergens - toxicity ; Animals ; Aspergillus niger ; Aspergillus niger - drug effects ; Aspergillus niger - growth &amp; development ; Biodiesel fuel ; BIOLOGY ; Calcium Compounds - pharmacology ; Cell Death - drug effects ; Cell Degranulation - drug effects ; Chlorocebus aethiops ; Enzyme Activation ; Fermentation ; Inactivation, Metabolic ; L-Lactate Dehydrogenase - metabolism ; Mast Cells - drug effects ; MEDICINE, RESEARCH &amp; EXPERIMENTAL ; Ricin ; Ricin - isolation &amp; purification ; Ricin - toxicity ; Ricinus communis ; Ricinus communis - drug effects ; Ricinus communis - toxicity ; Short Communication ; Solid-state fermentation ; Time Factors ; Toxicity Tests ; Vero cell ; Vero Cells</subject><ispartof>Brazilian journal of medical and biological research, 2012-11, Vol.45 (11), p.1002-1010</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383</citedby><cites>FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,24150,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22911344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernandes, K V</creatorcontrib><creatorcontrib>Deus-de-Oliveira, N</creatorcontrib><creatorcontrib>Godoy, M G</creatorcontrib><creatorcontrib>Guimarães, Z A S</creatorcontrib><creatorcontrib>Nascimento, V V</creatorcontrib><creatorcontrib>Melo, E J T de</creatorcontrib><creatorcontrib>Freire, D M G</creatorcontrib><creatorcontrib>Dansa-Petretski, M</creatorcontrib><creatorcontrib>Machado, O L T</creatorcontrib><title>Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds</title><title>Brazilian journal of medical and biological research</title><addtitle>Braz J Med Biol Res</addtitle><description>Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 x 10(5) cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH)2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.</description><subject>2S albumin</subject><subject>2S Albumins, Plant - isolation &amp; purification</subject><subject>2S Albumins, Plant - toxicity</subject><subject>Allergens - drug effects</subject><subject>Allergens - toxicity</subject><subject>Animals</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - drug effects</subject><subject>Aspergillus niger - growth &amp; development</subject><subject>Biodiesel fuel</subject><subject>BIOLOGY</subject><subject>Calcium Compounds - pharmacology</subject><subject>Cell Death - drug effects</subject><subject>Cell Degranulation - drug effects</subject><subject>Chlorocebus aethiops</subject><subject>Enzyme Activation</subject><subject>Fermentation</subject><subject>Inactivation, Metabolic</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Mast Cells - drug effects</subject><subject>MEDICINE, RESEARCH &amp; EXPERIMENTAL</subject><subject>Ricin</subject><subject>Ricin - isolation &amp; purification</subject><subject>Ricin - toxicity</subject><subject>Ricinus communis</subject><subject>Ricinus communis - drug effects</subject><subject>Ricinus communis - toxicity</subject><subject>Short Communication</subject><subject>Solid-state fermentation</subject><subject>Time Factors</subject><subject>Toxicity Tests</subject><subject>Vero cell</subject><subject>Vero Cells</subject><issn>0100-879X</issn><issn>1414-431X</issn><issn>1414-431X</issn><issn>0100-879X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNplUsFu1DAQjRCILoVfgBy5pIxjexNfkFBVoFIlDgWpN8t2Jlsvjr3YTqF_j7dZFipOtsfz3rx5M1X1hsAZ4QLeXQMBaPpO3LRAWoCOAxDaPqlWhBHWMEpunlarY9JJ9SKlLUDLgZHn1UnbCkIoY6tqurbT7LLyGOZUK-cwbtDX1iuT7Z3KNvha-aEeMIdfdrRmCYWxNirlEGuNypf7d6z1fZ0jqjyhz_VPm29L2Bk7T7UJ0y7Mfkgvq2ejcglfHc7T6tvHi6_nn5urL58uzz9cNYZznps16whdCySt7gkgb7XiqvSyHvoOtGICNHYawYyMma7vRuAClQFOsSNIe3paXS68Q1BbuYt2UvFeBmXlQyDEjVQxW-NQwsBMi0LgWhRDQOlh0IIVL3U3kgGwcJ0tXMlYdEFuwxx9ES8fZiD_zoCUZ7G4AN4vgN2sJxxMsSMq90jF4x9vb-Um3Enac0b4Xv3bA0EMP2ZMWU42GXRumZIkjPecllL7Wt2SamJIKeJ4LENA7jflP5WHTSnI1_-qPOL-rAb9DUoLuag</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Fernandes, K V</creator><creator>Deus-de-Oliveira, N</creator><creator>Godoy, M G</creator><creator>Guimarães, Z A S</creator><creator>Nascimento, V V</creator><creator>Melo, E J T de</creator><creator>Freire, D M G</creator><creator>Dansa-Petretski, M</creator><creator>Machado, O L T</creator><general>Sociedade Brasileira de Medicina Tropical</general><general>Associação Brasileira de Divulgação Científica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>H94</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20121101</creationdate><title>Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds</title><author>Fernandes, K V ; Deus-de-Oliveira, N ; Godoy, M G ; Guimarães, Z A S ; Nascimento, V V ; Melo, E J T de ; Freire, D M G ; Dansa-Petretski, M ; Machado, O L T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>2S albumin</topic><topic>2S Albumins, Plant - isolation &amp; purification</topic><topic>2S Albumins, Plant - toxicity</topic><topic>Allergens - drug effects</topic><topic>Allergens - toxicity</topic><topic>Animals</topic><topic>Aspergillus niger</topic><topic>Aspergillus niger - drug effects</topic><topic>Aspergillus niger - growth &amp; development</topic><topic>Biodiesel fuel</topic><topic>BIOLOGY</topic><topic>Calcium Compounds - pharmacology</topic><topic>Cell Death - drug effects</topic><topic>Cell Degranulation - drug effects</topic><topic>Chlorocebus aethiops</topic><topic>Enzyme Activation</topic><topic>Fermentation</topic><topic>Inactivation, Metabolic</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Mast Cells - drug effects</topic><topic>MEDICINE, RESEARCH &amp; EXPERIMENTAL</topic><topic>Ricin</topic><topic>Ricin - isolation &amp; purification</topic><topic>Ricin - toxicity</topic><topic>Ricinus communis</topic><topic>Ricinus communis - drug effects</topic><topic>Ricinus communis - toxicity</topic><topic>Short Communication</topic><topic>Solid-state fermentation</topic><topic>Time Factors</topic><topic>Toxicity Tests</topic><topic>Vero cell</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandes, K V</creatorcontrib><creatorcontrib>Deus-de-Oliveira, N</creatorcontrib><creatorcontrib>Godoy, M G</creatorcontrib><creatorcontrib>Guimarães, Z A S</creatorcontrib><creatorcontrib>Nascimento, V V</creatorcontrib><creatorcontrib>Melo, E J T de</creatorcontrib><creatorcontrib>Freire, D M G</creatorcontrib><creatorcontrib>Dansa-Petretski, M</creatorcontrib><creatorcontrib>Machado, O L T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Brazilian journal of medical and biological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandes, K V</au><au>Deus-de-Oliveira, N</au><au>Godoy, M G</au><au>Guimarães, Z A S</au><au>Nascimento, V V</au><au>Melo, E J T de</au><au>Freire, D M G</au><au>Dansa-Petretski, M</au><au>Machado, O L T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds</atitle><jtitle>Brazilian journal of medical and biological research</jtitle><addtitle>Braz J Med Biol Res</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>45</volume><issue>11</issue><spage>1002</spage><epage>1010</epage><pages>1002-1010</pages><issn>0100-879X</issn><issn>1414-431X</issn><eissn>1414-431X</eissn><eissn>0100-879X</eissn><abstract>Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 x 10(5) cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH)2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Medicina Tropical</pub><pmid>22911344</pmid><doi>10.1590/S0100-879X2012007500132</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0100-879X
ispartof Brazilian journal of medical and biological research, 2012-11, Vol.45 (11), p.1002-1010
issn 0100-879X
1414-431X
1414-431X
0100-879X
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_0d4c2e99e693440abddb94132b7f1d0e
source SciELO Brazil; IngentaConnect Journals
subjects 2S albumin
2S Albumins, Plant - isolation & purification
2S Albumins, Plant - toxicity
Allergens - drug effects
Allergens - toxicity
Animals
Aspergillus niger
Aspergillus niger - drug effects
Aspergillus niger - growth & development
Biodiesel fuel
BIOLOGY
Calcium Compounds - pharmacology
Cell Death - drug effects
Cell Degranulation - drug effects
Chlorocebus aethiops
Enzyme Activation
Fermentation
Inactivation, Metabolic
L-Lactate Dehydrogenase - metabolism
Mast Cells - drug effects
MEDICINE, RESEARCH & EXPERIMENTAL
Ricin
Ricin - isolation & purification
Ricin - toxicity
Ricinus communis
Ricinus communis - drug effects
Ricinus communis - toxicity
Short Communication
Solid-state fermentation
Time Factors
Toxicity Tests
Vero cell
Vero Cells
title Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T16%3A26%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20allergen%20inactivation%20and%20detoxification%20of%20castor%20bean%20cake%20by%20treatment%20with%20calcium%20compounds&rft.jtitle=Brazilian%20journal%20of%20medical%20and%20biological%20research&rft.au=Fernandes,%20K%20V&rft.date=2012-11-01&rft.volume=45&rft.issue=11&rft.spage=1002&rft.epage=1010&rft.pages=1002-1010&rft.issn=0100-879X&rft.eissn=1414-431X&rft_id=info:doi/10.1590/S0100-879X2012007500132&rft_dat=%3Cproquest_doaj_%3E1458530022%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c555t-6471369e12b810e52ba5a1006d870ba490be7be0cf44c787f059eac053e71e383%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1458530022&rft_id=info:pmid/22911344&rft_scielo_id=S0100_879X2012001100002&rfr_iscdi=true