LVING reveals the intracellular structure of cell growth

The continuous balance of growth and degradation inside cells maintains homeostasis. Disturbance of this balance by internal or external factors cause state of disease, while effective disease treatments seek to restore this balance. Here, we present a method based on quantitative phase imaging (QPI...

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Bibliographic Details
Published in:Scientific reports 2024-04, Vol.14 (1), p.8544-8544, Article 8544
Main Authors: Pradeep, Soorya, Zangle, Thomas A.
Format: Article
Language:English
Subjects:
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Autophagy
Biodegradation
Cell Cycle
Cell Division
Cell growth
Cell Proliferation
Chemotherapy
Homeostasis
Humanities and Social Sciences
Intracellular
Mass transport
Medical treatment
multidisciplinary
Receptor Protein-Tyrosine Kinases
Science
Science (multidisciplinary)
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Summary:The continuous balance of growth and degradation inside cells maintains homeostasis. Disturbance of this balance by internal or external factors cause state of disease, while effective disease treatments seek to restore this balance. Here, we present a method based on quantitative phase imaging (QPI) based measurements of cell mass and the velocity of mass transport to quantify the balance of growth and degradation within intracellular control volumes. The result, which we call Lagrangian velocimetry for intracellular net growth (LVING), provides high resolution maps of intracellular biomass production and degradation. We use LVING to quantify the growth in different regions of the cell during phases of the cell cycle. LVING can also be used to quantitatively compare the effect of range of chemotherapy drug doses on subcellular growth processes. Finally, we applied LVING to characterize the effect of autophagy on the growth machinery inside cells. Overall, LVING reveals both the structure and distribution of basal growth within cells, as well as the disruptions to this structure that occur during alterations in cell state.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-58992-x