Effective Degradation of Free Gossypol in Defatted Cottonseed Meal by Bacterial Laccases: Performance and Toxicity Analysis

Cottonseed meal (CSM) is the major by-product of the cottonseed oil extraction process with high protein content. However, the presence of free gossypol (FG) in CSM severely restricts its utilization in the food and animal feed industries. The development of a biological strategy for the effective r...

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Published in:Foods 2024-02, Vol.13 (4), p.566
Main Authors: Zhang, Liangyu, Zheng, Hao, Zhang, Xingke, Chen, Xiaoxue, Liu, Yanrong, Tang, Yu, Zhang, Wei, Wang, Zhixiang, Zhao, Lihong, Guo, Yongpeng
Format: Article
Language:English
Subjects:
Animal feed
Biocatalysts
Biocompatibility
Chromatography
Cottonseed meal
Degradation
Detoxification
E coli
Enzymes
Escherichia coli
Feed industry
Food industry
free gossypol
Gossypol
Hepatotoxicity
In vivo methods and tests
Laccase
Oils & fats
Oxidases
Proteins
Sodium
Sperm
Temperature effects
Toxicity
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Summary:Cottonseed meal (CSM) is the major by-product of the cottonseed oil extraction process with high protein content. However, the presence of free gossypol (FG) in CSM severely restricts its utilization in the food and animal feed industries. The development of a biological strategy for the effective removal of FG in CSM has become an urgent need. In this study, three bacterial laccases including CotA from , CueO from , and LcLac from were heterologously expressed and investigated for their FG degradation ability. The results showed that CotA laccase displayed the highest FG-degrading capacity among the three laccases, achieving 100% FG degradation at 37 °C and pH 7.0 in 1 h without the addition of a redox mediator. Moreover, in vitro and in vivo studies confirmed that the hepatotoxicity of FG was effectively eliminated after oxidative degradation by CotA laccase. Furthermore, the addition of CotA laccase could achieve 87% to 98% FG degradation in defatted CSM within 2 h. In conclusion, CotA laccase can be developed as an effective biocatalyst for the detoxification of FG in CSM.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods13040566