Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo

• Published in: Current stem cell research and therapy 2018-05, Vol.9 (1), p.120-13, Article 120
• Main Authors: Mena, Hebe Agustina, Zubiry, Paula Romina, Dizier, Blandine, Schattner, Mirta, Boisson-Vidal, Catherine, Negrotto, Soledad
• Format: Article
• Language: English
• Subjects: Acidic preconditioning; Angiogenesis; Care and treatment; Cellular Biology; Cellular therapy; Hind limb ischemia; Human endothelial colony forming cells; Inflammation; Life Sciences; Neovascularization; Type 2 diabetes
• ISSN: 1757-6512; 1757-6512; 1574-888X
• DOI: 10.1186/s13287-018-0872-7

We have previously demonstrated that acidic preconditioning of human endothelial colony-forming cells (ECFC) increased proliferation, migration, and tubulogenesis in vitro, and increased their regenerative potential in a murine model of hind limb ischemia without baseline disease. We now analyze whether this strategy is also effective under adverse conditions for vasculogenesis, such as the presence of ischemia-related toxic molecules or diabetes, one of the main target diseases for cell therapy due to their well-known healing impairments. Cord blood-derived CD34 cells were seeded in endothelial growth culture medium (EGM2) and ECFC colonies were obtained after 14-21 days. ECFC were exposed at pH 6.6 (preconditioned) or pH 7.4 (nonpreconditioned) for 6 h, and then pH was restored at 7.4. A model of type 2 diabetes induced by a high-fat and high-sucrose diet was developed in nude mice and hind limb ischemia was induced in these animals by femoral artery ligation. A P value