Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics

A novel fluorescence-sensing pathway for epinephrine (EP) detection was investigated. The ceramic-based miniature biosensor was developed through the immobilization of an enzyme (laccase, tyrosinase) on a polymer-poly-(2,6-di([2,2'-bithiophen]-5-yl)-4-(5-hexylthiophen-2-yl)pyridine), based on l...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2020-03, Vol.20 (5), p.1429
Main Authors: Baluta, Sylwia, Malecha, Karol, Świst, Agnieszka, Cabaj, Joanna
Format: Article
Language:English
Subjects:
biosensor
epinephrine
inorganic dye
laccase
ltcc
optical sensor
tyrosinase
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Summary:A novel fluorescence-sensing pathway for epinephrine (EP) detection was investigated. The ceramic-based miniature biosensor was developed through the immobilization of an enzyme (laccase, tyrosinase) on a polymer-poly-(2,6-di([2,2'-bithiophen]-5-yl)-4-(5-hexylthiophen-2-yl)pyridine), based on low temperature cofired ceramics technology (LTCC). The detection procedure was based on the oxidation of the substrate, i.e., in the presence of the enzyme. An alternative enzyme-free system utilized the formation of a colorful complex between Fe ions and epinephrine molecules. With the optimized conditions, the analytical performance illustrated high sensitivity and selectivity in a broad linear range with a detection limit of 0.14-2.10 nM. Moreover, the strategy was successfully used for an EP injection test with labeled pharmacological samples.
ISSN:1424-8220
1424-8220
DOI:10.3390/s20051429