High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria

Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method present...

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Bibliographic Details
Published in:Bio-protocol 2021-05, Vol.11 (9), p.e4001-e4001
Main Authors: Gu, ShaoHua, Wan, Wen, Shao, ZhengYing, Zhong, Wei
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by these rhizosphere bacteria. This method is a modified version of the universal chrome azurol S (CAS) assay that uses a 96-channel manual pipetting workstation. By combining the liquid CAS assay with the multi-channel pipette workstation, high-throughput and rapid detection of siderophore production can be achieved. In summary, this method can be used to gain a general understanding of siderophore production by rhizosphere bacteria.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.4001