Enhanced Ricinoleic Acid Preparation Using Lipozyme TLIM as a Novel Biocatalyst: Optimized by Response Surface Methodology

Ricinoleic acid (RA) is an important raw material for plasticizers, emulsifiers, and nanomaterials. In this work, a green and efficient method was developed for RA production. Results showed that Lipozyme TLIM can be used as a novel biocatalyst to catalyze the hydrolysis of castor oil (CO) for RA pr...

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Bibliographic Details
Published in:Catalysts 2018-11, Vol.8 (11), p.486
Main Authors: Sun, Shangde, Guo, Jingjing
Format: Article
Language:English
Subjects:
Biocatalysts
Carbon monoxide
Castor oil
Catalysts
Chemical reactions
Enzymes
Fatty acids
Hydrolysis
lipase
Load
Nanomaterials
Pancreas
Response surface methodology
Ricinoleic acid
Viscosity
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Summary:Ricinoleic acid (RA) is an important raw material for plasticizers, emulsifiers, and nanomaterials. In this work, a green and efficient method was developed for RA production. Results showed that Lipozyme TLIM can be used as a novel biocatalyst to catalyze the hydrolysis of castor oil (CO) for RA preparation. Response surface methodology (RSM) was used to evaluate and optimize the effects of reaction variables on the hydrolysis of CO. Reaction conditions were optimized as follows: 41.3 °C, enzyme load 8.9%, 39.2 h, and 40:1 molar ratio of water to oil. Under these optimized reaction variables, the maximum hydrolysis ratio of CO (96.2 ± 1.5%) was obtained. The effect of hydrolysis variables on the reaction was as follows: enzyme load > hydrolysis time > temperature. In conclusion, this is a green, simple, and efficient method for RA preparation and can provide a good alternative method for RA industrial production.
ISSN:2073-4344
2073-4344
DOI:10.3390/catal8110486