Novel Soloxolone Amides as Potent Anti-Glioblastoma Candidates: Design, Synthesis, In Silico Analysis and Biological Activities In Vitro and In Vivo

• Published in: Pharmaceuticals (Basel, Switzerland) Switzerland), 2022-05, Vol.15 (5), p.603
• Main Authors: Markov, Andrey V, Ilyina, Anna A, Salomatina, Oksana V, Sen'kova, Aleksandra V, Okhina, Alina A, Rogachev, Artem D, Salakhutdinov, Nariman F, Zenkova, Marina A
• Format: Article
• Language: English
• Subjects: 18βH-glycyrrhetinic acid; Acids; amides; Apoptosis; bardoxolone methyl; blood–brain barrier; Brain cancer; Cell cycle; Chromatography; Cytotoxicity; Fibroblasts; glioblastoma; Melanoma; Neuroblastoma; NMR; Nuclear magnetic resonance; Permeability; soloxolone methyl
• ISSN: 1424-8247; 1424-8247
• DOI: 10.3390/ph15050603

The modification of natural or semisynthetic triterpenoids with amines can be explored as a promising strategy for improving their pharmacological properties. Here, we report the design and synthesis of 11 novel amide derivatives of soloxolone methyl ( ), a cyano enone-bearing derivative of 18βH-glycyrrhetinic acid. Analysis of their bioactivities in vitro and in silico revealed their high toxicity against a panel of tumor cells (average IC = 3.7 µM) and showed that the formation of amide moieties at the C-30 position of soloxolone did not enhance the cytotoxicity of derivatives toward tumor cells compared to , though it can impart an ability to pass across the blood-brain barrier. Further HPLC-MS/MS and mechanistic studies verified significant brain accumulation of hit compound (soloxolone tryptamide) in a murine model and showed its high anti-glioblastoma potential. It was found that induced ROS-dependent and autophagy-independent death of U87 and U118 glioblastoma cells via mitochondrial apoptosis and effectively blocked their clonogenicity, motility and capacity to form vessel-like structures. Further in vivo study demonstrated that intraperitoneal injection of at a dosage of 20 mg/kg effectively inhibited the growth of U87 glioblastoma in a mouse xenograft model, reducing the proliferative potential of the tumor and leading to a depletion of collagen content and normalization of blood vessels in tumor tissue. The obtained results clearly demonstrate that can be considered as a promising leading compound for drug development in glioblastoma treatment.