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The ZmbHLH47-ZmSnRK2.9 Module Promotes Drought Tolerance in Maize
Drought stress globally poses a significant threat to maize ( L.) productivity and the underlying molecular mechanisms of drought tolerance remain elusive. In this study, we characterized ZmbHLH47, a basic helix-loop-helix (bHLH) transcription factor, as a positive regulator of drought tolerance in...
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| Published in: | International journal of molecular sciences 2024-05, Vol.25 (9), p.4957 |
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| container_issue | 9 |
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| container_title | International journal of molecular sciences |
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| creator | Yan, Zhenwei Zhang, Fajun Mu, Chunhua Ma, Changle Yao, Guoqi Sun, Yue Hou, Jing Leng, Bingying Liu, Xia |
| description | Drought stress globally poses a significant threat to maize (
L.) productivity and the underlying molecular mechanisms of drought tolerance remain elusive. In this study, we characterized ZmbHLH47, a basic helix-loop-helix (bHLH) transcription factor, as a positive regulator of drought tolerance in maize.
expression was notably induced by both drought stress and abscisic acid (ABA). Transgenic plants overexpressing
displayed elevated drought tolerance and ABA responsiveness, while the
mutant exhibited increased drought sensitivity and reduced ABA sensitivity. Mechanistically, it was revealed that ZmbHLH47 could directly bind to the promoter of
gene, a member of the subgroup III SnRK2 kinases, activating its expression. Furthermore,
-overexpressing plants exhibited enhanced ABA sensitivity and drought tolerance, whereas the
mutant displayed a decreased sensitivity to both. Notably, overexpressing
in the
mutant closely resembled the
mutant, indicating the importance of the ZmbHLH47-ZmSnRK2.9 module in ABA response and drought tolerance. These findings provided valuable insights and a potential genetic resource for enhancing the environmental adaptability of maize. |
| doi_str_mv | 10.3390/ijms25094957 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_0e3707f7a0e7478ca806eeef1cf17ba6</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A793564843</galeid><doaj_id>oai_doaj_org_article_0e3707f7a0e7478ca806eeef1cf17ba6</doaj_id><sourcerecordid>A793564843</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-7e83d77d1da1039d4bda226cc47fe8da25c4d6b42b5107c29f7276b848bac5bd3</originalsourceid><addsrcrecordid>eNpdkU1v1DAQhi0EoqVw44wiceFAtv7MxMdVKWzFVkXtcuklcuzJ1qskbu3kUH49brdUFZrDjEbPvOPxS8hHRhdCaHrsd0PiimqpFbwih0xyXlJawesX9QF5l9KOUi640m_JgahBcAbqkCw3N1hcD-1qvZJQXg9X4-VPvtDFeXBzj8WvGIYwYSq-xTBvb6ZiE3qMZrRY-LE4N_4PvidvOtMn_PCUj8jv76ebk1W5vvhxdrJcl1YyPZWAtXAAjjnDqNBOts5wXlkrocM618pKV7WSt4pRsFx3wKFqa1m3xqrWiSNyttd1weya2-gHE--bYHzz2Ahx25g4edtjQ1EAhQ4MRZBQW1PTChE7ZjsGramy1pe91m0MdzOmqRl8stj3ZsQwp0ZQJTSAknVGP_-H7sIcx3zpI5U_USmRqcWe2pq8349dmKKxORwO3oYRO5_7S9BCVbKWDwNf9wM2hpQids8XMdo8-Nq89DXjn55eMbcDumf4n5HiL7r_mrk</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3053175553</pqid></control><display><type>article</type><title>The ZmbHLH47-ZmSnRK2.9 Module Promotes Drought Tolerance in Maize</title><source>Open Access: PubMed Central</source><source>ProQuest - Publicly Available Content Database</source><creator>Yan, Zhenwei ; Zhang, Fajun ; Mu, Chunhua ; Ma, Changle ; Yao, Guoqi ; Sun, Yue ; Hou, Jing ; Leng, Bingying ; Liu, Xia</creator><creatorcontrib>Yan, Zhenwei ; Zhang, Fajun ; Mu, Chunhua ; Ma, Changle ; Yao, Guoqi ; Sun, Yue ; Hou, Jing ; Leng, Bingying ; Liu, Xia</creatorcontrib><description>Drought stress globally poses a significant threat to maize (
L.) productivity and the underlying molecular mechanisms of drought tolerance remain elusive. In this study, we characterized ZmbHLH47, a basic helix-loop-helix (bHLH) transcription factor, as a positive regulator of drought tolerance in maize.
expression was notably induced by both drought stress and abscisic acid (ABA). Transgenic plants overexpressing
displayed elevated drought tolerance and ABA responsiveness, while the
mutant exhibited increased drought sensitivity and reduced ABA sensitivity. Mechanistically, it was revealed that ZmbHLH47 could directly bind to the promoter of
gene, a member of the subgroup III SnRK2 kinases, activating its expression. Furthermore,
-overexpressing plants exhibited enhanced ABA sensitivity and drought tolerance, whereas the
mutant displayed a decreased sensitivity to both. Notably, overexpressing
in the
mutant closely resembled the
mutant, indicating the importance of the ZmbHLH47-ZmSnRK2.9 module in ABA response and drought tolerance. These findings provided valuable insights and a potential genetic resource for enhancing the environmental adaptability of maize.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25094957</identifier><identifier>PMID: 38732175</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>ABA response ; Abiotic stress ; Abscisic acid ; Abscisic Acid - metabolism ; Abscisic Acid - pharmacology ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; Corn ; CRISPR ; Drought ; Drought Resistance ; drought tolerance ; Droughts ; Gene Expression Regulation, Plant ; Genes ; Genetic engineering ; Genetically modified plants ; Genomes ; Kinases ; Localization ; maize ; Phosphatase ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Polypeptides ; Proteins ; Rice ; Signal transduction ; Stress, Physiological - genetics ; Transcription factors ; Zea mays - genetics ; Zea mays - metabolism ; Zea mays - physiology ; ZmbHLH47 ; ZmSnRK2.9</subject><ispartof>International journal of molecular sciences, 2024-05, Vol.25 (9), p.4957</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c419t-7e83d77d1da1039d4bda226cc47fe8da25c4d6b42b5107c29f7276b848bac5bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3053175553/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3053175553?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38732175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Zhenwei</creatorcontrib><creatorcontrib>Zhang, Fajun</creatorcontrib><creatorcontrib>Mu, Chunhua</creatorcontrib><creatorcontrib>Ma, Changle</creatorcontrib><creatorcontrib>Yao, Guoqi</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Hou, Jing</creatorcontrib><creatorcontrib>Leng, Bingying</creatorcontrib><creatorcontrib>Liu, Xia</creatorcontrib><title>The ZmbHLH47-ZmSnRK2.9 Module Promotes Drought Tolerance in Maize</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Drought stress globally poses a significant threat to maize (
L.) productivity and the underlying molecular mechanisms of drought tolerance remain elusive. In this study, we characterized ZmbHLH47, a basic helix-loop-helix (bHLH) transcription factor, as a positive regulator of drought tolerance in maize.
expression was notably induced by both drought stress and abscisic acid (ABA). Transgenic plants overexpressing
displayed elevated drought tolerance and ABA responsiveness, while the
mutant exhibited increased drought sensitivity and reduced ABA sensitivity. Mechanistically, it was revealed that ZmbHLH47 could directly bind to the promoter of
gene, a member of the subgroup III SnRK2 kinases, activating its expression. Furthermore,
-overexpressing plants exhibited enhanced ABA sensitivity and drought tolerance, whereas the
mutant displayed a decreased sensitivity to both. Notably, overexpressing
in the
mutant closely resembled the
mutant, indicating the importance of the ZmbHLH47-ZmSnRK2.9 module in ABA response and drought tolerance. These findings provided valuable insights and a potential genetic resource for enhancing the environmental adaptability of maize.</description><subject>ABA response</subject><subject>Abiotic stress</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Abscisic Acid - pharmacology</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>Corn</subject><subject>CRISPR</subject><subject>Drought</subject><subject>Drought Resistance</subject><subject>drought tolerance</subject><subject>Droughts</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetically modified plants</subject><subject>Genomes</subject><subject>Kinases</subject><subject>Localization</subject><subject>maize</subject><subject>Phosphatase</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Polypeptides</subject><subject>Proteins</subject><subject>Rice</subject><subject>Signal transduction</subject><subject>Stress, Physiological - genetics</subject><subject>Transcription factors</subject><subject>Zea mays - genetics</subject><subject>Zea mays - metabolism</subject><subject>Zea mays - physiology</subject><subject>ZmbHLH47</subject><subject>ZmSnRK2.9</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkU1v1DAQhi0EoqVw44wiceFAtv7MxMdVKWzFVkXtcuklcuzJ1qskbu3kUH49brdUFZrDjEbPvOPxS8hHRhdCaHrsd0PiimqpFbwih0xyXlJawesX9QF5l9KOUi640m_JgahBcAbqkCw3N1hcD-1qvZJQXg9X4-VPvtDFeXBzj8WvGIYwYSq-xTBvb6ZiE3qMZrRY-LE4N_4PvidvOtMn_PCUj8jv76ebk1W5vvhxdrJcl1YyPZWAtXAAjjnDqNBOts5wXlkrocM618pKV7WSt4pRsFx3wKFqa1m3xqrWiSNyttd1weya2-gHE--bYHzz2Ahx25g4edtjQ1EAhQ4MRZBQW1PTChE7ZjsGramy1pe91m0MdzOmqRl8stj3ZsQwp0ZQJTSAknVGP_-H7sIcx3zpI5U_USmRqcWe2pq8349dmKKxORwO3oYRO5_7S9BCVbKWDwNf9wM2hpQids8XMdo8-Nq89DXjn55eMbcDumf4n5HiL7r_mrk</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Yan, Zhenwei</creator><creator>Zhang, Fajun</creator><creator>Mu, Chunhua</creator><creator>Ma, Changle</creator><creator>Yao, Guoqi</creator><creator>Sun, Yue</creator><creator>Hou, Jing</creator><creator>Leng, Bingying</creator><creator>Liu, Xia</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20240501</creationdate><title>The ZmbHLH47-ZmSnRK2.9 Module Promotes Drought Tolerance in Maize</title><author>Yan, Zhenwei ; 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L.) productivity and the underlying molecular mechanisms of drought tolerance remain elusive. In this study, we characterized ZmbHLH47, a basic helix-loop-helix (bHLH) transcription factor, as a positive regulator of drought tolerance in maize.
expression was notably induced by both drought stress and abscisic acid (ABA). Transgenic plants overexpressing
displayed elevated drought tolerance and ABA responsiveness, while the
mutant exhibited increased drought sensitivity and reduced ABA sensitivity. Mechanistically, it was revealed that ZmbHLH47 could directly bind to the promoter of
gene, a member of the subgroup III SnRK2 kinases, activating its expression. Furthermore,
-overexpressing plants exhibited enhanced ABA sensitivity and drought tolerance, whereas the
mutant displayed a decreased sensitivity to both. Notably, overexpressing
in the
mutant closely resembled the
mutant, indicating the importance of the ZmbHLH47-ZmSnRK2.9 module in ABA response and drought tolerance. These findings provided valuable insights and a potential genetic resource for enhancing the environmental adaptability of maize.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38732175</pmid><doi>10.3390/ijms25094957</doi><oa>free_for_read</oa></addata></record> |
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| subjects | ABA response Abiotic stress Abscisic acid Abscisic Acid - metabolism Abscisic Acid - pharmacology Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - metabolism Corn CRISPR Drought Drought Resistance drought tolerance Droughts Gene Expression Regulation, Plant Genes Genetic engineering Genetically modified plants Genomes Kinases Localization maize Phosphatase Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Polypeptides Proteins Rice Signal transduction Stress, Physiological - genetics Transcription factors Zea mays - genetics Zea mays - metabolism Zea mays - physiology ZmbHLH47 ZmSnRK2.9 |
| title | The ZmbHLH47-ZmSnRK2.9 Module Promotes Drought Tolerance in Maize |
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