Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer

• Published in: Journal of pharmacy & pharmacognosy research 2022-07, Vol.10 (4), p.595-604
• Main Authors: Muti’ah, Roihatul, Endharti, Agustina T., Wafi, Muhammad F.
• Format: Article
• Language: English
• Subjects: 1.4-naphtoquinone; dayak onion; immunohistochemistry
• ISSN: 0719-4250; 0719-4250
• DOI: 10.56499/jppres21.1321.10.4.595

Context: Medicinal plants can be used as an option for the prevention and reduction of cancer cell resistance and its side effects. Sisyrinchium palmifolium L. is thought to have anti-cancer activity with a compound content of 1,4-naphthoquinone. Aims: To determine the effect of S. palmifolium extract (SPE) with the main compound 1,4-naphthoquinone on Ki-67 expression by in vivo, and CDK1, CDK2, and CDK4 activity by in silico in colonic epithelial cells of BALB/ c mice induced by azoxymethane (AOM) dextran sodium sulfate (DSS). Methods: Dayak onion (S. palmifolium) was extracted using 96% ethanol as a solvent. The S. palmifolium extract was then made into tablet form by the wet granulation method. Mice that had been induced with AOM-DSS were given S. palmifolium extract therapy. Twenty samples were used, which were divided into five groups. Mice colon tissue was assessed using Ki-67 immunohistochemistry. This study also used the in silico method to see the effect of 1,4-naphthoquinone compounds from S. palmifolium extract on the expression of CDK1, CDK2 and CDK4 with PDB codes 6GU6, 6GUC, and 1GIH. Results: Ki-67 expression values were 26 ± 6.51 cells at low dosages, 15 ± 1.73 cells at moderate doses, and 11 ± 1.04 cells at high doses. Between the test groups, there was a statistical differences (p