Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer

Context: Medicinal plants can be used as an option for the prevention and reduction of cancer cell resistance and its side effects. Sisyrinchium palmifolium L. is thought to have anti-cancer activity with a compound content of 1,4-naphthoquinone. Aims: To determine the effect of S. palmifolium extra...

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Published in:Journal of pharmacy & pharmacognosy research 2022-07, Vol.10 (4), p.595-604
Main Authors: Muti’ah, Roihatul, Endharti, Agustina T., Wafi, Muhammad F.
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1.4-naphtoquinone
dayak onion
immunohistochemistry
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description Context: Medicinal plants can be used as an option for the prevention and reduction of cancer cell resistance and its side effects. Sisyrinchium palmifolium L. is thought to have anti-cancer activity with a compound content of 1,4-naphthoquinone. Aims: To determine the effect of S. palmifolium extract (SPE) with the main compound 1,4-naphthoquinone on Ki-67 expression by in vivo, and CDK1, CDK2, and CDK4 activity by in silico in colonic epithelial cells of BALB/ c mice induced by azoxymethane (AOM) dextran sodium sulfate (DSS). Methods: Dayak onion (S. palmifolium) was extracted using 96% ethanol as a solvent. The S. palmifolium extract was then made into tablet form by the wet granulation method. Mice that had been induced with AOM-DSS were given S. palmifolium extract therapy. Twenty samples were used, which were divided into five groups. Mice colon tissue was assessed using Ki-67 immunohistochemistry. This study also used the in silico method to see the effect of 1,4-naphthoquinone compounds from S. palmifolium extract on the expression of CDK1, CDK2 and CDK4 with PDB codes 6GU6, 6GUC, and 1GIH. Results: Ki-67 expression values were 26 ± 6.51 cells at low dosages, 15 ± 1.73 cells at moderate doses, and 11 ± 1.04 cells at high doses. Between the test groups, there was a statistical differences (p
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Sisyrinchium palmifolium L. is thought to have anti-cancer activity with a compound content of 1,4-naphthoquinone. Aims: To determine the effect of S. palmifolium extract (SPE) with the main compound 1,4-naphthoquinone on Ki-67 expression by in vivo, and CDK1, CDK2, and CDK4 activity by in silico in colonic epithelial cells of BALB/ c mice induced by azoxymethane (AOM) dextran sodium sulfate (DSS). Methods: Dayak onion (S. palmifolium) was extracted using 96% ethanol as a solvent. The S. palmifolium extract was then made into tablet form by the wet granulation method. Mice that had been induced with AOM-DSS were given S. palmifolium extract therapy. Twenty samples were used, which were divided into five groups. Mice colon tissue was assessed using Ki-67 immunohistochemistry. This study also used the in silico method to see the effect of 1,4-naphthoquinone compounds from S. palmifolium extract on the expression of CDK1, CDK2 and CDK4 with PDB codes 6GU6, 6GUC, and 1GIH. Results: Ki-67 expression values were 26 ± 6.51 cells at low dosages, 15 ± 1.73 cells at moderate doses, and 11 ± 1.04 cells at high doses. Between the test groups, there was a statistical differences (p&lt;0.05) with the Post Hoc Mann-Whitney test. At the 6GUC receptor, the mean rerank score of the 1,4-naphtoquinone molecule, which was closest to the native ligand, was -54.6572 ± 2.2722 and -90.5455 ± 1.6524kcal/mole. The steric bond on the amino acid lys 33 (A), which exclusively occurs at the 6GUC receptor, was the only commonality of contact. Conclusions: 1,4-Naphthoquinone from Sisyrinchium palmifolium L. extract could decrease Ki-67 expression by in vivo, which cloud induce a decrease in epithelial cells proliferation in colon cancer, but has no potential as an inhibitor activity of CDK1, CDK2, and CDK4 by in silico.</description><identifier>ISSN: 0719-4250</identifier><identifier>EISSN: 0719-4250</identifier><identifier>DOI: 10.56499/jppres21.1321.10.4.595</identifier><language>eng</language><publisher>GarVal Editorial Ltda</publisher><subject>1.4-naphtoquinone ; dayak onion ; immunohistochemistry</subject><ispartof>Journal of pharmacy &amp; pharmacognosy research, 2022-07, Vol.10 (4), p.595-604</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Muti’ah, Roihatul</creatorcontrib><creatorcontrib>Endharti, Agustina T.</creatorcontrib><creatorcontrib>Wafi, Muhammad F.</creatorcontrib><title>Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer</title><title>Journal of pharmacy &amp; pharmacognosy research</title><description>Context: Medicinal plants can be used as an option for the prevention and reduction of cancer cell resistance and its side effects. Sisyrinchium palmifolium L. is thought to have anti-cancer activity with a compound content of 1,4-naphthoquinone. Aims: To determine the effect of S. palmifolium extract (SPE) with the main compound 1,4-naphthoquinone on Ki-67 expression by in vivo, and CDK1, CDK2, and CDK4 activity by in silico in colonic epithelial cells of BALB/ c mice induced by azoxymethane (AOM) dextran sodium sulfate (DSS). Methods: Dayak onion (S. palmifolium) was extracted using 96% ethanol as a solvent. The S. palmifolium extract was then made into tablet form by the wet granulation method. Mice that had been induced with AOM-DSS were given S. palmifolium extract therapy. Twenty samples were used, which were divided into five groups. Mice colon tissue was assessed using Ki-67 immunohistochemistry. This study also used the in silico method to see the effect of 1,4-naphthoquinone compounds from S. palmifolium extract on the expression of CDK1, CDK2 and CDK4 with PDB codes 6GU6, 6GUC, and 1GIH. Results: Ki-67 expression values were 26 ± 6.51 cells at low dosages, 15 ± 1.73 cells at moderate doses, and 11 ± 1.04 cells at high doses. Between the test groups, there was a statistical differences (p&lt;0.05) with the Post Hoc Mann-Whitney test. At the 6GUC receptor, the mean rerank score of the 1,4-naphtoquinone molecule, which was closest to the native ligand, was -54.6572 ± 2.2722 and -90.5455 ± 1.6524kcal/mole. The steric bond on the amino acid lys 33 (A), which exclusively occurs at the 6GUC receptor, was the only commonality of contact. Conclusions: 1,4-Naphthoquinone from Sisyrinchium palmifolium L. extract could decrease Ki-67 expression by in vivo, which cloud induce a decrease in epithelial cells proliferation in colon cancer, but has no potential as an inhibitor activity of CDK1, CDK2, and CDK4 by in silico.</description><subject>1.4-naphtoquinone</subject><subject>dayak onion</subject><subject>immunohistochemistry</subject><issn>0719-4250</issn><issn>0719-4250</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpNkd1KxDAQhYsoKOozmAewNWkmSXMp6y8ueKFehzRN3CzdpiZV9Hl8UZv1B2_ODOcwHwOnKE4IrhgHKc_W4xhtqklFaBZcQcUk2ykOsCCyhJrh3X_7fnGc0hpjTAQHKshB8XnpnDUTCg6RUygHPa6mVXh59UMYLHIxbNCDTx_RD2blXzdo1P3Gu9DnfVkh-z5Fnc8H5Af05t8CuvMlF3OQ_0p-DvTQ5TD53puAFhd35DRrvVXIp2bmTT6VOqVgvJ5sl60c6MHYeFTsOd0ne_wzD4unq8vHxU25vL--XZwvS0M4ZSUFx6HRjEhtcNualhPJpMSCCytYXTvRtARaI7BgFBPNuJRNBxYYlZ0zDT0sbr-5XdBrNUa_0fFDBe3V1gjxWek4edNbhS2Yxs0VWAAwFjdSutZRaBsrBOv4zBLfLBNDStG6Px7Balud-q1O5eqyDWqujn4B5H-NsQ</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Muti’ah, Roihatul</creator><creator>Endharti, Agustina T.</creator><creator>Wafi, Muhammad F.</creator><general>GarVal Editorial Ltda</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>20220701</creationdate><title>Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer</title><author>Muti’ah, Roihatul ; Endharti, Agustina T. ; Wafi, Muhammad F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1635-34f648a519ac0bbcb6195990767e7522f78b14bc7075301a56998d4e4539dfc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>1.4-naphtoquinone</topic><topic>dayak onion</topic><topic>immunohistochemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muti’ah, Roihatul</creatorcontrib><creatorcontrib>Endharti, Agustina T.</creatorcontrib><creatorcontrib>Wafi, Muhammad F.</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of pharmacy &amp; pharmacognosy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muti’ah, Roihatul</au><au>Endharti, Agustina T.</au><au>Wafi, Muhammad F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer</atitle><jtitle>Journal of pharmacy &amp; pharmacognosy research</jtitle><date>2022-07-01</date><risdate>2022</risdate><volume>10</volume><issue>4</issue><spage>595</spage><epage>604</epage><pages>595-604</pages><issn>0719-4250</issn><eissn>0719-4250</eissn><abstract>Context: Medicinal plants can be used as an option for the prevention and reduction of cancer cell resistance and its side effects. Sisyrinchium palmifolium L. is thought to have anti-cancer activity with a compound content of 1,4-naphthoquinone. Aims: To determine the effect of S. palmifolium extract (SPE) with the main compound 1,4-naphthoquinone on Ki-67 expression by in vivo, and CDK1, CDK2, and CDK4 activity by in silico in colonic epithelial cells of BALB/ c mice induced by azoxymethane (AOM) dextran sodium sulfate (DSS). Methods: Dayak onion (S. palmifolium) was extracted using 96% ethanol as a solvent. The S. palmifolium extract was then made into tablet form by the wet granulation method. Mice that had been induced with AOM-DSS were given S. palmifolium extract therapy. Twenty samples were used, which were divided into five groups. Mice colon tissue was assessed using Ki-67 immunohistochemistry. This study also used the in silico method to see the effect of 1,4-naphthoquinone compounds from S. palmifolium extract on the expression of CDK1, CDK2 and CDK4 with PDB codes 6GU6, 6GUC, and 1GIH. Results: Ki-67 expression values were 26 ± 6.51 cells at low dosages, 15 ± 1.73 cells at moderate doses, and 11 ± 1.04 cells at high doses. Between the test groups, there was a statistical differences (p&lt;0.05) with the Post Hoc Mann-Whitney test. At the 6GUC receptor, the mean rerank score of the 1,4-naphtoquinone molecule, which was closest to the native ligand, was -54.6572 ± 2.2722 and -90.5455 ± 1.6524kcal/mole. The steric bond on the amino acid lys 33 (A), which exclusively occurs at the 6GUC receptor, was the only commonality of contact. Conclusions: 1,4-Naphthoquinone from Sisyrinchium palmifolium L. extract could decrease Ki-67 expression by in vivo, which cloud induce a decrease in epithelial cells proliferation in colon cancer, but has no potential as an inhibitor activity of CDK1, CDK2, and CDK4 by in silico.</abstract><pub>GarVal Editorial Ltda</pub><doi>10.56499/jppres21.1321.10.4.595</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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subjects 1.4-naphtoquinone
dayak onion
immunohistochemistry
title Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer
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