DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis

Specimens of have been collected from alfalfa fields in Kearny County, Kansas & Carbon County, Montana. DNA barcoding with the COI mitochondrial gene indicate that the species is not , soybean cyst nematode, , sugar beet cyst nematode, or , clover cyst nematode. Maximum likelihood phylogenetic t...

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Bibliographic Details
Published in:Journal of nematology 2019-01, Vol.51 (1), p.1-17
Main Authors: Powers, Thomas, Skantar, Andrea, Harris, Tim, Higgins, Rebecca, Mullin, Peter, Hafez, Saad, Handoo, Zafar, Todd, Tim, Powers, Kirsten
Format: Article
Language:English
Subjects:
Alfalfa cyst nematode
COI DNA barcode
Detection
Diagnosis
heterodera medicaginis
Life Sciences
Taxonomy
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Summary:Specimens of have been collected from alfalfa fields in Kearny County, Kansas & Carbon County, Montana. DNA barcoding with the COI mitochondrial gene indicate that the species is not , soybean cyst nematode, , sugar beet cyst nematode, or , clover cyst nematode. Maximum likelihood phylogenetic trees show that the alfalfa specimens form a sister clade most closely related to , with a 4.7% mean pairwise sequence divergence across the 862 nucleotides of the COI marker. Morphological analyses of juveniles and cysts conform to the measurements of , the alfalfa cyst nematode originally described from the USSR in 1971. Initial host testing demonstrated that the nematode reproduced on alfalfa, but not on soybeans, tomato, or corn. Collectively, the evidence suggests that this finding represents the first record of in North America. Definitive confirmation of this diagnosis would require COI sequence of eastern European isolates of this species.
ISSN:0022-300X
2640-396X
2640-396X
DOI:10.21307/jofnem-2019-016