Candida albicans and non- albicans Isolates from Bloodstream Have Different Capacities to Induce Neutrophil Extracellular Traps

• Published in: Journal of fungi (Basel) 2019-04, Vol.5 (2), p.28
• Main Authors: Campos-Garcia, Lizbeth, Jimenez-Valdes, Rocio Jimena, Hernandez-Bello, Romel, Palma-Nicolas, Jose, Gonzalez, Gloria Maria, Sanchez-Gonzalez, Alejandro
• Format: Article
• Language: English
• Subjects: Blood & organ donations; Candida albicans; Candida glabrata; Candida non-albicans; Fungi; Hyphae; Kinases; Leukocytes (neutrophilic); neutrophil extracellular traps; Neutrophils; Nosocomial infections; Participation; Pathogens; Phospholipase; phospholipases; Species; Virulence
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof5020028

Neutrophils activated with pathogens or their products induce formation of extracellular traps (NETs), but if this constitutes a general response against all pathogenic species in a single genus or intrageneric differences exist remains unknown, yet this is of great importance for the establishment of effective treatments. To determine this, we analyzed neutrophil extracellular traps formation after the stimulation with bloodstream isolates from different species ( , , , and ), and found that each species has a different capacity to induce DNA extrusion, which is independent of their morphology (yeast or hyphae). We observed that phospholipase producer's strains and their secretion products were able to induce NETs, a property not observed with phospholipase deficient strains, with exception of some sensu stricto isolates, which showed no NETs induction although they did show phospholipase production. To further analyze this, we extended our study to include cryptic species ( and ) and no extracellular traps formation was observed. Here, we contribute to the understanding of how neutrophils initiate NETs, and we found that certain strains may have a differential capacity to trigger these structures, which may explain the high mortality of some isolates.