Isolation of Microsatellite Markers in a Chaparral Species Endemic to Southern California, Ceanothus megacarpus (Rhamnaceae)

Premise of the study: Microsatellite (simple sequence repeat [SSR]) markers were developed for Ceanothus megacarpus, a chaparral species endemic to coastal southern California, to investigate potential processes (e.g., fragmentation, genetic drift, and interspecific hybridization) responsible for th...

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Bibliographic Details
Published in:Applications in plant sciences 2013-05, Vol.1 (5), p.1200393-n/a
Main Authors: Ishibashi, Caitlin D. A, Shaver, Anthony R, Perrault, David P, Davis, Stephen D, Honeycutt, Rodney L
Format: Article
Language:English
Subjects:
alleles
California
Ceanothus
Ceanothus megacarpus
Chaparral
Cloning
DNA primers
Endemic species
Genetic diversity
Genetic drift
Genetic markers
Genetic structure
genetic variation
Heterozygosity
Hybridization
indigenous species
Interspecific
Interspecific hybridization
loci
microsatellite repeats
Microsatellites
Phylogenetics
Plant sciences
Polymorphism
population
PRIMER NOTE
Rhamnaceae
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Summary:Premise of the study: Microsatellite (simple sequence repeat [SSR]) markers were developed for Ceanothus megacarpus, a chaparral species endemic to coastal southern California, to investigate potential processes (e.g., fragmentation, genetic drift, and interspecific hybridization) responsible for the genetic structure within and among populations distributed throughout mainland and island populations. Methods and Results: Four SSR-enriched libraries were used to develop and optimize 10 primer sets of microsatellite loci containing either di-, tri-, or tetranucleotide repeats. Levels of variation at these loci were assessed for two populations of C. megacarpus. Observed heterozygosity ranged from 0.250 to 0.885, and number of alleles ranged between four and 21 per locus. Eight to nine loci also successfully amplified in three other species of Ceanothus. Conclusions: These markers should prove useful for evaluating the influence of recent and historical processes on genetic variation in C. megacarpus and related species.
ISSN:2168-0450
2168-0450
DOI:10.3732/apps.1200393