Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis

Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was used to compare the composition of ginsenosides in white ginseng (WG) and extruded white ginseng (EWG). A total of 45 saponins, including original neutral ginsenosides, malonyl-ginse...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2020, Vol.2020 (2020), p.1-9
Main Authors: Liu, Jing-Sheng, Wu, Yu-Zhu, Zheng, Ming-Zhu, Zhao, Cheng-Bin, Zhang, Hao, Xu, Xiu-Ying, Zhao, Yi-Lin, Wang, Yang, Guo, Yun-Long, Liu, Shu-Ying
Format: Article
Language:English
Subjects:
Chromatography
Cluster analysis
Clustering
Composition
Extrusion
Gas flow
Ions
Liquid chromatography
Mass spectrometry
Medicine
Multivariate analysis
Multivariate statistical analysis
Quadrupoles
Saponins
Software
Statistical analysis
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Summary:Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was used to compare the composition of ginsenosides in white ginseng (WG) and extruded white ginseng (EWG). A total of 45 saponins, including original neutral ginsenosides, malonyl-ginsenosides, and chemical transformation of ginsenosides, were successfully identified in both WG and EWG. Multivariate statistical analyses including supervised orthogonal partial least squared discrimination analysis (OPLS-DA) and hierarchical clustering analysis (HCA) were used to analyze components of white ginseng before and after extrusion. As a result, three ginsenosides (malonyl (M)-Rb1, M-Rb2, and M-Rc) were found to be increased in WG, while three ginsenosides (Rb2, Rc, and Rg1) were elevated in EWG. In the OPLS-DA S-plot, the different compositions of ginsenoside that were distinguished between WG and EWG were screened out. Experimental results indicate that the UHPLC-Q-Orbitrap-MS/MS is a useful tool to characterize variations of ginsenosides in WG and EWG.
ISSN:2090-8865
2090-8873
DOI:10.1155/2020/4764219