Comparative detection efficacy of primers targeting SpeI-AvaI restriction fragment and small subunit ribosomal RNA gene of Babesia bigemina

The objective of the study is to evaluate the comparative detection efficacy of primers targeting SpeI-AvaI restriction fragment and small subunit ribosomal RNA (SSU rRNA) gene of Babesia bigemina by employing conventional polymerase chain reaction (PCR) on 783 animals (296 cattle and 487 buffaloes)...

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Bibliographic Details
Published in:Indian journal of animal sciences 2023-08, Vol.92 (7)
Main Authors: KAUR, PARAMJIT, JUYAL, PRAYAG DUTT, SHARMA, AMRITA, SINGLA, LACHHMAN DAS, MUKHOPADHYAY, CHANDRA SEKHAR
Format: Article
Language:English
Subjects:
Babesia bigemina, PCR, SpeI-AvaI restriction fragment, SSU rRNA gene
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Summary:The objective of the study is to evaluate the comparative detection efficacy of primers targeting SpeI-AvaI restriction fragment and small subunit ribosomal RNA (SSU rRNA) gene of Babesia bigemina by employing conventional polymerase chain reaction (PCR) on 783 animals (296 cattle and 487 buffaloes) of low lying (bet) area of Punjab. The detection rate of SpeI-AvaI and SSU rRNA PCR assays was 3.96% (31/783), and 6.64% (52/783), respectively. Among cattle and buffaloes, prevalence of B. bigemina was higher (P
ISSN:0367-8318
2394-3327
DOI:10.56093/ijans.v92i7.109919