Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane

Enzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In t...

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Bibliographic Details
Published in:Catalysts 2019-06, Vol.9 (6), p.495
Main Authors: Chen, Peng-Cheng, Ma, Zhen, Zhu, Xue-Yan, Chen, Da-Jing, Huang, Xiao-Jun
Format: Article
Language:English
Subjects:
Acids
Adsorption
Aqueous solutions
Bioreactors
Catalysis
Catalysts
Chemical reactions
Efficiency
enzymatic membrane bioreactor
Enzymes
Feed direction
filtration
gradient-pore membrane
Hollow fiber membranes
Hydrolysates
hydrolysis
Immobilization
Influence
Lipase
Membranes
Microfiltration
Morphology
Optimization
Parameters
Permeability
Polysulfone resins
Pore size
Substrates
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Summary:Enzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In this work, an EMBR based on hollow fiber polysulfone microfiltration membranes with radial gradient pore structure was fabricated and enzyme immobilization was achieved through pressure-driven filtration. Lipase from Candida rugosa was used for immobilization and EMBR performance was studied with the enzymatic hydrolysis of glycerol triacetate as a model reaction. The influences of membrane pore diameter, substrate feed direction as well as operational parameters of operation pressure, substrate concentration, and temperature on the EMBR activity were investigated with the production of hydrolysates kinetically fitted. The complete EMBR system showed the highest activity of 1.07 × 104 U⋅g−1. The results in this work indicate future efforts for improvement in EMBR.
ISSN:2073-4344
2073-4344
DOI:10.3390/catal9060495