Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia

Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Method...

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Published in:Biomedicines 2021-11, Vol.9 (11), p.1715
Main Authors: Santiago-Fernández, Concepción, Martín-Reyes, Flores, Tome, Monica, Gutierrez-Repiso, Carolina, Fernandez-Garcia, Diego, Ocaña-Wilhelmi, Luis, Rivas-Becerra, Jose, Tatzber, Franz, Pursch, Edith, Tinahones, Francisco J., García-Fuentes, Eduardo, Garrido-Sánchez, Lourdes
Format: Article
Language:English
Subjects:
Adipocytes
Atherosclerosis
Biobanks
Body fat
Body mass index
CXCL16 protein
Diabetes
Disease
Gene expression
Hospitals
Hypoxia
Hypoxia-inducible factor 1a
Inflammation
Insulin resistance
Interleukin 6
Low density lipoprotein
LOX-1 protein
Metabolism
morbid obesity
Obesity
oxidized LDL
Scavenger receptors
Statistical analysis
Tumor necrosis factor-α
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Summary:Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Methods: We studied in 17 non-obese and 20 subjects with morbid obesity (MO) the mRNA expression of HIF-1α, SRs (LOX-1, MSR1, CL-P1 and CXCL16), IL6 and TNFα in visceral adipocytes and the effect of hypoxia with or without ox-LDL on visceral in vitro-differentiated adipocytes (VDA). Results: HIF-1α, TNFα, IL6, LOX-1, MSR1 and CXCL16 expression in adipocytes was increased in MO when compared with those in non-obese subjects (p < 0.05). The expression of most of the inflammatory markers and SRs gene correlated with HIF-1α. In VDA, hypoxia increased TNFα, IL6, MSR1, CXCL16 and CL-P1 (p < 0.05) in non-obese subjects, and TNFα, IL6, MSR1 and CXCL16 (p < 0.05) in MO. Silencing HIF-1α prevented the increase of TNFα, IL6, LOX-1, MSR1, CL-P1 and CXCL16 expression (p < 0.05). The combination of hypoxia and ox-LDL produced higher TNFα expression (p = 0.041). Conclusions: Morbid obesity and hypoxia increased SRs and inflammatory markers in visceral adipocytes. In a hypoxic state, ox-LDL increased the proinflammatory response of visceral adipocytes to hypoxia.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9111715