PTD-FNK Alleviated LPS-Induced Oxidative Stress of Boar Testicular Sertoli Cells via Keap1-Nrf2 Pathway

PTD-FNK, a synthetic anti-apoptotic protein, has been shown to potently alleviate cellular injuries. However, the effects of PTD-FNK on oxidative defense in boar testicular Sertoli cells (SCs) against oxidative injury has not been explored. In this study, we show that exposure of SCs to 100 mg/L lip...

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Published in:Veterinary sciences 2024-11, Vol.11 (11), p.543
Main Authors: Ji, Weixia, Huang, Qiuyan, Ma, Qiqi, Song, Xingxing, Zhang, Xin, Li, Xun, Wang, Xiaoye, Wang, Sutian, Wang, Yanling, Xiao, Zhengzhong, Hu, Chuanhuo
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Blood
Cell death
Enzymatic activity
Enzymes
Genes
Health aspects
Homeostasis
Lipopolysaccharides
Nrf2 pathway
Oxidative stress
Proteins
PTD-FNK
Reagents
Reproductive system
Sertoli cell
Sertoli cells
Signal transduction
Sperm
Spermatogenesis
Testes
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Summary:PTD-FNK, a synthetic anti-apoptotic protein, has been shown to potently alleviate cellular injuries. However, the effects of PTD-FNK on oxidative defense in boar testicular Sertoli cells (SCs) against oxidative injury has not been explored. In this study, we show that exposure of SCs to 100 mg/L lipopolysaccharide (LPS) for 12 h leads to decreased survival rate, superoxide dismutase (SOD) activity, and increased malondialdehyde (MDA). Treatment with 0.01 nmol/L PTD-FNK for 4 h significantly enhanced the activity of SOD, catalase (CAT), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in SCs. Concurrently, PTD-FNK treatment effectively reduced the production of reactive oxygen species (ROS) and the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in SCs. Moreover, using His pull-down and LC-MS techniques, we identified PTD-FNK-interacting proteins and confirmed that this protective effect may be mediated by the regulation of the - signaling pathway by PTD-FNK. Therefore, PTD-FNK alleviates LPS-induced oxidative stress via the / pathway, providing novel insights for the development of therapeutic agents targeting testicular oxidative damage.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci11110543