Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids

• Published in: Foods 2020-07, Vol.9 (7), p.934
• Main Authors: Martínez, Tomás Francisco, Alcalde, María Jesús, Sáez, María Isabel, Suárez, María Dolores
• Format: Article
• Language: English
• Subjects: Aging; Agricultural practices; Animal young; Calcium-binding protein; Compliance; Councils; Dehydrogenases; Electrophoresis; Farm management; Farms; Food science; Gel electrophoresis; goat kids; Goats; Kinases; Meat; Meat quality; Metabolism; Metabolites; Methods; Muscle proteins; Muscles; myofibrillar protein; Physiological aspects; Physiology; Polyacrylamide; Proteins; sarcoplasmic protein; SDS-PAGE; Sodium dodecyl sulfate; Sodium lauryl sulfate; Stress; Suckling behavior; transport; Transportation; Troponin; Troponin T
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods9070934

The combined effect of farm management practices, transport time, and ageing time on the electrophoretic changes of sarcoplasmic (SPP) and myofibrillar (MFP) protein fractions of goat kids was studied. A total of 64 suckling goat kids were withdrawn from two farms with "high" (GW) and "low" (DW) welfare-friendly management practices, and they were transported for 2 or 6 h immediately before slaughtering. samples were obtained at 3, 8, and 21 days post-mortem, and muscle proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE. Both protein extracts displayed significant changes attributable to meat maturation. Managing conditions of kids in DW farms increased the post-mortem susceptibility of muscle proteins. Some MFP of muscle, such as troponin T, as well as 26-30 and 35-37 kDa fractions were influenced significantly by deficient on-farm management, and therefore, these protein fragments might be considered as indicators of low-welfare on-farm management in goat kids.