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Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids
The combined effect of farm management practices, transport time, and ageing time on the electrophoretic changes of sarcoplasmic (SPP) and myofibrillar (MFP) protein fractions of goat kids was studied. A total of 64 suckling goat kids were withdrawn from two farms with "high" (GW) and &quo...
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Published in: | Foods 2020-07, Vol.9 (7), p.934 |
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description | The combined effect of farm management practices, transport time, and ageing time on the electrophoretic changes of sarcoplasmic (SPP) and myofibrillar (MFP) protein fractions of goat kids was studied. A total of 64 suckling goat kids were withdrawn from two farms with "high" (GW) and "low" (DW) welfare-friendly management practices, and they were transported for 2 or 6 h immediately before slaughtering.
samples were obtained at 3, 8, and 21 days post-mortem, and muscle proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE. Both protein extracts displayed significant changes attributable to meat maturation. Managing conditions of kids in DW farms increased the post-mortem susceptibility of muscle proteins. Some MFP of
muscle, such as troponin T, as well as 26-30 and 35-37 kDa fractions were influenced significantly by deficient on-farm management, and therefore, these protein fragments might be considered as indicators of low-welfare on-farm management in goat kids. |
doi_str_mv | 10.3390/foods9070934 |
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samples were obtained at 3, 8, and 21 days post-mortem, and muscle proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE. Both protein extracts displayed significant changes attributable to meat maturation. Managing conditions of kids in DW farms increased the post-mortem susceptibility of muscle proteins. Some MFP of
muscle, such as troponin T, as well as 26-30 and 35-37 kDa fractions were influenced significantly by deficient on-farm management, and therefore, these protein fragments might be considered as indicators of low-welfare on-farm management in goat kids.</description><identifier>ISSN: 2304-8158</identifier><identifier>EISSN: 2304-8158</identifier><identifier>DOI: 10.3390/foods9070934</identifier><identifier>PMID: 32679760</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Aging ; Agricultural practices ; Animal young ; Calcium-binding protein ; Compliance ; Councils ; Dehydrogenases ; Electrophoresis ; Farm management ; Farms ; Food science ; Gel electrophoresis ; goat kids ; Goats ; Kinases ; Meat ; Meat quality ; Metabolism ; Metabolites ; Methods ; Muscle proteins ; Muscles ; myofibrillar protein ; Physiological aspects ; Physiology ; Polyacrylamide ; Proteins ; sarcoplasmic protein ; SDS-PAGE ; Sodium dodecyl sulfate ; Sodium lauryl sulfate ; Stress ; Suckling behavior ; transport ; Transportation ; Troponin ; Troponin T</subject><ispartof>Foods, 2020-07, Vol.9 (7), p.934</ispartof><rights>COPYRIGHT 2020 MDPI AG</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-93ab62d93d99392e85d5be2747a0ed66f1a3ef57dc59f12b8d3a9e9de5a9b6103</citedby><cites>FETCH-LOGICAL-c506t-93ab62d93d99392e85d5be2747a0ed66f1a3ef57dc59f12b8d3a9e9de5a9b6103</cites><orcidid>0000-0002-3468-9681 ; 0000-0003-1944-4499</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2425021252/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2425021252?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32679760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martínez, Tomás Francisco</creatorcontrib><creatorcontrib>Alcalde, María Jesús</creatorcontrib><creatorcontrib>Sáez, María Isabel</creatorcontrib><creatorcontrib>Suárez, María Dolores</creatorcontrib><title>Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids</title><title>Foods</title><addtitle>Foods</addtitle><description>The combined effect of farm management practices, transport time, and ageing time on the electrophoretic changes of sarcoplasmic (SPP) and myofibrillar (MFP) protein fractions of goat kids was studied. A total of 64 suckling goat kids were withdrawn from two farms with "high" (GW) and "low" (DW) welfare-friendly management practices, and they were transported for 2 or 6 h immediately before slaughtering.
samples were obtained at 3, 8, and 21 days post-mortem, and muscle proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE. Both protein extracts displayed significant changes attributable to meat maturation. Managing conditions of kids in DW farms increased the post-mortem susceptibility of muscle proteins. Some MFP of
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A total of 64 suckling goat kids were withdrawn from two farms with "high" (GW) and "low" (DW) welfare-friendly management practices, and they were transported for 2 or 6 h immediately before slaughtering.
samples were obtained at 3, 8, and 21 days post-mortem, and muscle proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE. Both protein extracts displayed significant changes attributable to meat maturation. Managing conditions of kids in DW farms increased the post-mortem susceptibility of muscle proteins. Some MFP of
muscle, such as troponin T, as well as 26-30 and 35-37 kDa fractions were influenced significantly by deficient on-farm management, and therefore, these protein fragments might be considered as indicators of low-welfare on-farm management in goat kids.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32679760</pmid><doi>10.3390/foods9070934</doi><orcidid>https://orcid.org/0000-0002-3468-9681</orcidid><orcidid>https://orcid.org/0000-0003-1944-4499</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Aging Agricultural practices Animal young Calcium-binding protein Compliance Councils Dehydrogenases Electrophoresis Farm management Farms Food science Gel electrophoresis goat kids Goats Kinases Meat Meat quality Metabolism Metabolites Methods Muscle proteins Muscles myofibrillar protein Physiological aspects Physiology Polyacrylamide Proteins sarcoplasmic protein SDS-PAGE Sodium dodecyl sulfate Sodium lauryl sulfate Stress Suckling behavior transport Transportation Troponin Troponin T |
title | Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids |
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