Comparative Transcriptome Profiling of Gaeumannomyces graminis var. tritici in Wheat Roots in the Absence and Presence of Biocontrol Bacillus velezensis CC09

• Published in: Frontiers in microbiology 2019-07, Vol.10, p.1474-1474
• Main Authors: Kang, Xingxing, Guo, Yu, Leng, Shuang, Xiao, Lei, Wang, Lanhua, Xue, Yarong, Liu, Changhong
• Format: Article
• Language: English
• Subjects: endophytic bacteria; Microbiology; pathogenic fungi; phytopathology; RNA sequencing; wheat disease
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.01474

This study aimed to explore potential biocontrol mechanisms involved in the interference of antagonistic bacteria with fungal pathogenicity . To do this, we conducted a comparative transcriptomic analysis of the "take-all" pathogenic fungus var. ( ) by examining -infected wheat roots in the presence or absence of the biocontrol agent CC09 ( ) compared with grown on potato dextrose agar (PDA) plates. A total of 4,134 differentially expressed genes (DEGs) were identified in -infected wheat roots, while 2,011 DEGs were detected in + -infected roots, relative to the grown on PDA plates. Moreover, 31 DEGs were identified between wheat roots, respectively infected with and + , consisting of 29 downregulated genes coding for potential pathogenicity factors - e.g., para-nitrobenzyl esterase, cutinase 1 and catalase-3, and two upregulated genes coding for tyrosinase and a hypothetical protein in the + -infected roots when compared with the -infected roots. In particular, the expression of one gene, encoding the ABA3 involved in the production of 's hormone abscisic acid, was 4.11-fold lower in -infected roots with than without . This is the first experimental study to analyze the activity of transcriptomes in wheat roots exposed or not to a biocontrol bacterium. Our results therefore suggest the presence of directly and/or indirectly impairs the pathogenicity of in wheat roots through complex regulatory mechanisms, such as hyphopodia formation, cell wall hydrolase, and expression of a papain inhibitor, among others, all which merit further investigation.