Self-Healing Alginate Hydrogel Formed by Dynamic Benzoxaborolate Chemistry Protects Retinal Pigment Epithelium Cells against Oxidative Damage

Oxidative stress is considered as a major factor causing retinal pigment epithelium (RPE) dysfunction and finally leading to retinal diseases such as age-related macular degeneration (AMD). Developing hydrogels for RPE cell delivery, especially those with antioxidant feature, is emerging as a promis...

Full description

Saved in:
Bibliographic Details
Published in:Gels 2022-12, Vol.9 (1), p.24
Main Authors: Liu, Minhua, Huang, Yate, Tao, Chunwen, Yang, Weijia, Chen, Junrong, Zhu, Li, Pan, Tonghe, Narain, Ravin, Nan, Kaihui, Chen, Yangjun
Format: Article
Language:English
Subjects:
Age related diseases
alginate
Alginates
Antioxidants
Biocompatibility
Biomedical materials
Cell culture
Conjugation
Crosslinking
Damage
dynamic crosslinking
Epithelium
Eye diseases
Hydrogels
Macular degeneration
oxidative stress
Photoreceptors
Physiology
Polymers
Polysaccharides
Polyvinyl alcohol
Retina
retinal pigment epithelium
Rheology
self-healing hydrogel
Stem cells
Tissue engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxidative stress is considered as a major factor causing retinal pigment epithelium (RPE) dysfunction and finally leading to retinal diseases such as age-related macular degeneration (AMD). Developing hydrogels for RPE cell delivery, especially those with antioxidant feature, is emerging as a promising approach for AMD treatment. Herein, a readily prepared antioxidant alginate-based hydrogel was developed to serve as a cytoprotective agent for RPE cells against oxidative damage. Alg-BOB was synthesized via conjugation of benzoxaborole (BOB) to the polysaccharide backbone. Hydrogels were formed through self-crosslinking of Alg-BOB based on benzoxaborole-diol complexation. The resulting hydrogel showed porous micro-structure, pH dependent mechanical strength and excellent self-healing, remolding, and injectable properties. Moreover, the hydrogel exhibited excellent cytocompatibility and could efficiently scavenge reactive oxygen species (ROS) to achieve an enhanced viability of ARPE-19 cells under oxidative condition. Altogether, our study reveals that the antioxidant Alg-BOB hydrogel represents an eligible candidate for RPE delivery and AMD treatment.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels9010024