Nucleoli in large (giant) bi- and multinucleate cells after apoptosis-inducing photodynamic treatment

The present experimental study was undertaken to provide information on nucleolar changes accompanying the apoptotic process in large or giant binucleate and multinucleate cells (LBMNCs). Such cells were present in a small but constant percentage in cultures of HL-60 cells. The apoptotic process was...

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Bibliographic Details
Published in:European journal of histochemistry 2003-01, Vol.47 (1), p.39-43
Main Authors: Smetana, K, Hrkal, Z
Format: Article
Language:English
Subjects:
Aminolevulinic Acid - pharmacology
Apoptosis - drug effects
Cell Nucleolus - chemistry
Cell Nucleolus - drug effects
Cell Nucleolus - ultrastructure
Cell Survival - drug effects
Giant Cells - drug effects
Giant Cells - ultrastructure
Granulocytes - drug effects
Granulocytes - pathology
HL-60 Cells - drug effects
HL-60 Cells - pathology
Humans
Leukemia - drug therapy
Leukemia - pathology
Light
Nucleolus Organizer Region - chemistry
Nucleolus Organizer Region - drug effects
Nucleolus Organizer Region - ultrastructure
Photochemotherapy
Photosensitizing Agents - pharmacology
RNA - analysis
Silver Staining
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Summary:The present experimental study was undertaken to provide information on nucleolar changes accompanying the apoptotic process in large or giant binucleate and multinucleate cells (LBMNCs). Such cells were present in a small but constant percentage in cultures of HL-60 cells. The apoptotic process was induced by photodynamic treatment (PDT) by means of 5-aminolaevulinic acid (ALA) as the precursor of the photosensitizer protoporphyrin IX and irradiation with broad spectrum blue light (BL). Nucleolar changes in LBMNCs were characterized by marked reduction or disappearance of silver stained particles representing AgNORs in nucleoli including the large ones. In addition, PDT also significantly reduced the number of nucleoli regardless of their size. These changes apparently reflected the decrease or cessation of nucleolar biosynthetic activities and resembled those which were previously observed in naturally maturing bone marrow megakaryocytes (Janoutová et al., 2001).
ISSN:1121-760X
2038-8306
DOI:10.4081/805