Bariatric surgery can acutely modulate ER-stress and inflammation on subcutaneous adipose tissue in non-diabetic patients with obesity

• Published in: Diabetology and metabolic syndrome 2021-02, Vol.13 (1), p.19-19, Article 19
• Main Authors: Ferraz-Bannitz, Rafael, Welendorf, Caroline Rossi, Coelho, Priscila Oliveira, Salgado, Jr, Wilson, Nonino, Carla Barbosa, Beraldo, Rebeca A, Foss-Freitas, Maria Cristina
• Format: Article
• Language: English
• Subjects: Adipogenesis; Adiponectin; Adipose tissue; Adipose tissues; AMP; AMP-activated protein kinase; Bariatric surgery; Body fat; Body mass index; Body weight loss; Calreticulin; Comparative analysis; Diabetes; Diabetes mellitus; Diabetics; Dietary restrictions; Endoplasmic reticulum; Endoplasmic reticulum stress; Energy balance; Gastric bypass; Gastrointestinal surgery; Gene expression; Genes; Genetic translation; Homeostasis; Human adipose tissue; Inflammation; Initiation factor eIF-2; Insulin resistance; Interleukin 6; Interleukins; Kinases; Mitochondria; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Monocytes; Obesity; Oxidative stress; Patients; Physiological aspects; Protein kinases; Roux-en-Y gastric bypass; Sensors; Surgery; Type 2 diabetes
• ISSN: 1758-5996; 1758-5996
• DOI: 10.1186/s13098-021-00623-w

Bariatric surgery, especially Roux-en-Y gastric bypass (RYGB), is the most effective and durable treatment option for severe obesity. The mechanisms involving adipose tissue may be important to explain the effects of surgery. We aimed to identify the genetic signatures of adipose tissue in patients undergoing RYGB. We evaluated 13 obese, non-diabetic patients (mean age 37 years, 100% women, Body mass index (BMI) 42.2 kg/m ) one day before surgery, 3 and 6 months (M) after RYGB. Analysis of gene expression in adipose tissue collected at surgery compared with samples collected at 3 M and 6 M Post-RYGB showed that interleukins [Interleukin 6, Tumor necrosis factor-α (TNF-α), and Monocyte chemoattractant protein-1(MCP1)] and endoplasmic reticulum stress (ERS) genes [Eukaryotic translation initiation factor 2 alpha kinase 3 (EIF2AK3) and Calreticulin (CALR)] decreased during the follow-up (P ≤ 0.01 for all). Otherwise, genes involved in energy homeostasis [Adiponectin and AMP-activated protein kinase (AMPK)], cellular response to oxidative stress [Sirtuin 1, Sirtuin 3, and Nuclear factor erythroid 2-related factor 2 (NRF2)], mitochondrial biogenesis [Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α)] and amino acids metabolism [General control nonderepressible 2 (GCN2)] increased from baseline to all other time points evaluated (P ≤ 0.01 for all). Also, expression of Peroxisome proliferator-activated receptor gamma (PPARϒ) (adipogenesis regulation) was significantly decreased after RYGB (P