ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating ThWRKY4

Heat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of ThHSFA1-ThWRKY4 in Tamari...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-05, Vol.22 (9), p.5048
Main Authors: Sun, Ting-Ting, Wang, Chao, Liu, Rui, Zhang, Yu, Wang, Yu-Cheng, Wang, Liu-Qiang
Format: Article
Language:English
Subjects:
Abiotic stress
Abscisic acid
antioxidant enzyme
Antioxidants
C-Terminus
Chromatin
Cloning
Environmental impact
Enzymatic activity
Enzyme activity
Enzymes
Gene expression
Heat shock
heat shock element
heat shock transcription factor
Homeostasis
Immunoprecipitation
Localization
Physiology
Proteins
Reactive oxygen species
ROS
Salinity
Salinity tolerance
salt stress
Scavenging
Tamarix hispida
Tobacco
Transcription factors
Transgenic plants
Yeast
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Summary:Heat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of ThHSFA1-ThWRKY4 in Tamarix hispida and their functions and regulatory mechanisms in salt tolerance. ThHSFA1 protein acts as an upstream regulator that can directly activate ThWRKY4 expression by binding to the heat shock element (HSE) of the ThWRKY4 promoter using yeast one-hybrid (Y1H), chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays. ThHSFA1 and ThWRKY4 expression was significantly induced by salt stress and abscisic acid (ABA) treatment in the roots and leaves of T. hispida. ThHSFA1 is a nuclear-localized protein with transactivation activity at the C-terminus. Compared to nontransgenic plants, transgenic plants overexpressing ThHSFA1 displayed enhanced salt tolerance and exhibited reduced reactive oxygen species (ROS) levels and increased antioxidant enzyme activity levels under salt stress. Therefore, we further concluded that ThHSFA1 mediated the regulation of ThWRKY4 in response to salt stress in T. hispida.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22095048