Characterization and chromosomal mapping of the DgmarMITE transposon in populations of Dichotomius (Luederwaldtinia) sericeus species complex (Coleoptera: Scarabaeidae)

Transposable elements are dispersed repetitive DNA sequences that can move within the genome and are related to genome and chromosome evolution, adaptation, and speciation. The aim of this study was to characterize and determine the chromosomal location and accumulation of a Mariner-like element in...

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Bibliographic Details
Published in:Genetics and molecular biology 2018-04, Vol.41 (2), p.419-425
Main Authors: Amorim, Igor Costa, Costa, Rafaelle Grazielle Coelho, Xavier, Crislaine, Moura, Rita de Cássia de
Format: Article
Language:English
Subjects:
Accumulation
Animal Genetics
BIOCHEMISTRY & MOLECULAR BIOLOGY
Biological evolution
chromosome evolution
cross-mobilization
Deoxyribonucleic acid
DNA
Fluorescence
Fluorescence in situ hybridization
Gene mapping
Gene sequencing
GENETICS & HEREDITY
Genomes
Karyotypes
Mapping
Mariner-like elements
Nucleotide sequence
Phylogeny
Populations
Recombination
Speciation
Species
Transposase
Transposons
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Summary:Transposable elements are dispersed repetitive DNA sequences that can move within the genome and are related to genome and chromosome evolution, adaptation, and speciation. The aim of this study was to characterize and determine the chromosomal location and accumulation of a Mariner-like element in populations of four phylogenetically related species of the Dichotomius (Luederwaldtinia) sericeus complex. Mapping of the isolated element was performed by fluorescent in situ hybridization in different populations of analyzed species. Characterization of the isolated element revealed a degenerated transposon, named DgmarMITE. This transposon is 496-bp-long, AT rich (57%), and contains 24 bp terminal inverted repeats. In situ mapping revealed presence of this element only in two out of four species analyzed. DgmarMITE sites were located in heterochromatic and euchromatic regions and varied in location and number on the karyotypes of Dichotomius (L.) gilletti and D. (L.) guaribensis across different populations. These results demonstrate differential accumulation of the DgmarMITE in genomes of these species, which is probably due to the occurrence of ectopic recombination and cross-mobilization of the element mediated by the transposase of closely related or unrelated transposable elements.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-GMB-2017-0230