The BnaBPs gene regulates flowering time and leaf angle in Brassica napus

The flowering time and plant architecture of Brassica napus were significantly associated with yield. In this study, we found that the BREVIPEDICELLUS/KNAT1(BP) gene regulated the flowering time and plant architecture of B. napus. However, the precise regulatory mechanism remains unclear. We cloned...

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Bibliographic Details
Published in:Plant direct 2024-10, Vol.8 (10), p.e70018-n/a
Main Authors: Yu, Jiang, Xue, Yi‐Xuan, Sarwar, Rehman, Wei, Shi‐Hao, Geng, Rui, Zhang, Yan‐Feng, Mu, Jian‐Xin, Tan, Xiao‐Li
Format: Article
Language:English
Subjects:
Abscission
Agricultural production
Amino acid sequence
Biosynthesis
BnaBP
Brassica napus
Budding
Cloning
Conserved sequence
CRISPR
Crop yield
Ethylene
Flowering
flowering time
Genes
Germination
Gibberellins
KNOX protein family
Leaf angle
Leaves
Localization
Phenotypes
Phylogenetics
plant architecture
Plant growth
Proteins
Regulatory sequences
Rice
Sequence analysis
Transcription factors
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Summary:The flowering time and plant architecture of Brassica napus were significantly associated with yield. In this study, we found that the BREVIPEDICELLUS/KNAT1(BP) gene regulated the flowering time and plant architecture of B. napus. However, the precise regulatory mechanism remains unclear. We cloned two homologous BP genes, BnaBPA03 and BnaBPC03, from B. napus Xiaoyun. The protein sequence analysis showed two proteins containing conserved domains KNOX I, KNOX II, ELK, and HOX of the KONX protein family. The CRISPR/Cas9 knockout lines exhibited early budding and flowering time, coupled with floral organ abscission earlier and a larger leaf angle. On the contrary, overexpression plants displayed a phenotype that was the inverse of these characteristics. Furthermore, we observed upregulation of gibberellin and ethylene biosynthesis genes, as well as floral integrator genes in knocked‐out plants. The results revealed that BnaBPs play a role in flowering time, floral organ abscission, and leaf angle as well as germination processes mediated. Additionally, BnaBPs exerted an impact on the biosynthesis pathways of ethylene and GA.
ISSN:2475-4455
2475-4455
DOI:10.1002/pld3.70018