Specific populations of urinary extracellular vesicles and proteins differentiate type 1 primary hyperoxaluria patients without and with nephrocalcinosis or kidney stones

• Published in: Orphanet journal of rare diseases 2020-11, Vol.15 (1), p.319-319, Article 319
• Main Authors: Jayachandran, Muthuvel, Yuzhakov, Stanislav V, Kumar, Sanjay, Larson, Nicholas B, Enders, Felicity T, Milliner, Dawn S, Rule, Andrew D, Lieske, John C
• Format: Article
• Language: English
• Subjects: Age; Antibodies; Biological markers; Biomarkers; Calcification; Calcinosis; Calcium oxalate; Calcium phosphates; Care and treatment; CD14 antigen; CD40 antigen; Creatinine; Development and progression; E-cadherin; Endoglin; Epidermal growth factor receptors; Exosomes; Extracellular Vesicles; Female; Growth factors; Health aspects; Humans; Hyperoxaluria; Hyperoxaluria, Primary; Identification and classification; Inflammation; Intercellular adhesion molecule 1; Kidney Calculi; Kidney diseases; Kidney stones; Klotho protein; Laminin; Male; Medical research; Microvesicles; Monocyte chemoattractant protein 1; Morbidity; Mortality; Nephrocalcinosis; Nephrolithiasis; Neprilysin; Oxalate; Oxalic acid; Patients; Proteins; Rare diseases; Renal calcification; Statistical analysis; Urinary proteins; Urinary vesicles; Urine; β2 Microglobulin
• ISSN: 1750-1172; 1750-1172
• DOI: 10.1186/s13023-020-01607-1

Primary hyperoxaluria type 1 (PH1) is associated with nephrocalcinosis (NC) and calcium oxalate (CaOx) kidney stones (KS). Populations of urinary extracellular vesicles (EVs) can reflect kidney pathology. The aim of this study was to determine whether urinary EVs carrying specific biomarkers and proteins differ among PH1 patients with NC, KS or with neither disease process. Mayo Clinic Rare Kidney Stone Consortium bio-banked cell-free urine from male and female PH1 patients without (n = 10) and with NC (n = 6) or KS (n = 9) and an eGFR > 40 mL/min/1.73 m were studied. Urinary EVs were quantified by digital flow cytometer and results expressed as EVs/ mg creatinine. Expressions of urinary proteins were measured by customized antibody array and results expressed as relative intensity. Data were analyzed by ANCOVA adjusting for sex, and biomarkers differences were considered statistically significant among groups at a false discovery rate threshold of Q