Metabolomics and transcriptomics reveal the effect of hetero-chitooligosaccharides in promoting growth of Brassica napus

• Published in: Scientific reports 2022-12, Vol.12 (1), p.21197-21197, Article 21197
• Main Authors: Tang, Chao, Zhai, Yang, Wang, Zhuo, Zhao, Xin, Yang, Chen, Zhao, Yong, Zeng, Liang-bin, Zhang, De-yong
• Format: Article
• Language: English
• Subjects: 631/337; 631/443; Acetates; ARF protein; Biopolymers; Brassica napus; Brassica napus - genetics; Crustaceans; Exoskeleton; Humanities and Social Sciences; Indoleacetic acid; Metabolites; Metabolomics; multidisciplinary; Phytohormones; Plant growth; Plant Growth Regulators; Plant hormones; Plant resistance; Rape plants; Science; Science (multidisciplinary); Signal transduction; Transcriptomes; Transcriptomics; γ-Aminobutyric acid
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-25850-7

The hetero-chitooligosaccharide (HTCOS) is a naturally occurring biopolymer in the exoskeleton of crustaceans and insects. Although some studies have been carried out on HTCOS in inducing plant resistance and promoting growth, the molecular mechanism of HTCOS in plants is not clear. In this study, an integrated analysis of metabolomics and transcriptomics was performed to analyze the response of Brassica napus to hetero-chitooligosaccharides treatment. The levels of 26 metabolites in B. napus were significantly changed under the HTCOS treatment. Amongst these metabolites, 9 metabolites were significantly up-regulated, including pentonic acid, indole-3-acetate, and γ-aminobutyric acid. Transcriptome data showed that there were 817 significantly up-regulated genes and 1064 significantly down-regulated genes in B. napus under the HTCOS treatment. Interestingly, the indole-3-acetate (IAA) content under the HTCOS treatment was about five times higher than that under the control condition. Moreover, four genes related to plant hormone signal transduction, three AUX/IAA genes, and one ARF gene, were significantly up-regulated under the HTCOS treatment. Furthermore, the plant height, branching number, and biomass of B. napus under the HTCOS treatment were significantly increased compared to that in the control condition. This evidence indicated that the HTCOS treatment contributed to accumulating the content of plant hormone IAA in the B. napus , up-regulating the expression of key genes in the signaling pathway of plant growth and improving the agronomic traits of B. napus .