Identification of Mycoviruses in the Pathogens of Fragrant Pear Valsa Canker from Xinjiang in China

• Published in: Viruses 2024-02, Vol.16 (3), p.355
• Main Authors: Zhang, Chenguang, Zheng, Xiaoya, Tie, Zhanjiang, Xi, Hui, Shi, Mai, Ma, Yanjun, Chen, Wenbin, Mi, Yingjie, Yang, Rui, Zhao, Sifeng, Zhang, Xuekun
• Format: Article
• Language: English
• Subjects: Ascomycota; biocontrol; Bioinformatics; Biological control; Branches; Canker; Control; Disease prevention; Diseases and pests; Dominant species; fragrant pear canker disease; Fruits; Fungal Viruses - genetics; Fungi; Genetic testing; Growth rate; Identification and classification; Maximum likelihood method; metatranscriptomics; Morphology; mycovirus; Pathogens; Pear; Pesticides; Phomopsis; Phylogenetics; Plant diseases; Plant Diseases - microbiology; Plant viruses; Pyrus; RNA Viruses - genetics; Transfer RNA; Valsa spp; Virulence; Virus diseases of plants; Viruses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v16030355

As a common disease, canker seriously affects the yield and quality of fragrant pear due to the lack of effective control measures. Some fungi have been reported to harbor rich reservoirs of viral resources, and some mycoviruses can be used as biocontrol agents against plant diseases. In this study, 199 isolates were obtained from diseased branches of fragrant pear in the main production areas of Xinjiang. Among them, 134 belonged to spp., identified using morphological and molecular biological techniques, in which was the dominant species. The mycoviruses in spp. were further identified using metatranscriptomic sequencing and RT-PCR. The results revealed that a total of seven mycoviruses were identified, belonging to , , , , , and , among which Phomopsis longicolla hypovirus (PlHV) was dominant in all the sample collection regions. The Cryphonectria hypovirus 3-XJ1 (CHV3-XJ1), Botourmiaviridae sp.-XJ1 (BVsp-XJ1), and Fusariviridae sp.-XJ1 (Fvsp-XJ1) were new mycoviruses discovered within the spp. More importantly, compared with those in the virus-free spp. strain, the growth rate and virulence of the VN-5 strain co-infected with PlHV and CHV3-XJ1 were reduced by 59% and 75%, respectively, and the growth rate and virulence of the VN-34 strain infected with PlHV were reduced by 42% and 55%, respectively. On the other hand, the horizontal transmission efficiency of PlHV decreased when PlHV was co-infected with CHV3-XJ1, indicating that PlHV and CHV3-XJ1 were antagonistic. In summary, the mycoviruses in spp. were identified in Xinjiang for the first time, and three of them were newly discovered mycoviruses, with two strains yielding good results. These results will offer potential biocontrol resources for managing pear canker disease and provide a theoretical basis for the control of fruit tree Valsa canker disease.