Shear stress inhibits apoptosis of ischemic brain microvascular endothelial cells

As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protecti...

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Bibliographic Details
Published in:International journal of molecular sciences 2013-01, Vol.14 (1), p.1412-1427
Main Authors: Tian, Shan, Bai, Yulong, Yang, Lin, Wang, Xinggang, Wu, Yi, Jia, Jie, Zhu, Yulian, Cheng, Yong, Zhang, Pengyue, Wu, Junfa, Wang, Nianhong, Xia, Guang, Liao, Hua, Zhang, Yuling, Shen, Xiafeng, Yu, Huixian, Hu, Yongshan
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Blotting, Western
Brain - blood supply
Brain Ischemia
brain microvascular endothelial cells
Brain research
Cell cycle
Cells, Cultured
Electronic mail systems
Endothelial Cells - metabolism
Endothelium
Exercise
Flow velocity
Gene Expression
Hospitals
Ischemia
ischemic stroke
Kinases
laminar shear stress
Male
Membrane Potential, Mitochondrial - physiology
Mitochondria - metabolism
Mitochondria - physiology
Morphology
Neurosciences
Permeability
Proteins
Proto-Oncogene Proteins c-akt - genetics
Proto-Oncogene Proteins c-akt - metabolism
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
Rats, Sprague-Dawley
Receptor, TIE-2 - genetics
Receptor, TIE-2 - metabolism
Regional Blood Flow
Reverse Transcriptase Polymerase Chain Reaction
Shear Strength
Shear stress
Signal Transduction
Stress, Mechanical
Stroke
Tumor necrosis factor-TNF
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Summary:As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms14011412