Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria

• Published in: Veterinary World 2018-03, Vol.11 (3), p.373-378
• Main Authors: Manel, Merradi, Nouzha, Heleili, Rim, Mekari, Imane, Mekkaoui, Sana, Aouachria, Yasmine, Oucheriah, Ammar, Ayachi
• Format: Article
• Language: English
• Subjects: 1-diphenyl-2- picrylhydrazyl radical; Acetic acid; Antibacterial activity; Antibacterial agents; antioxidant activity; Antioxidants; Antioxidants (Nutrients); Bacteria; Biological activity; Biphenyl (Compound); Chloroform; Drug resistance; Enterobacter cloacae; Escherichia coli; Esters; Ethyl acetate; extraction; Gram-positive bacteria; Inhibition; Juniperus thurifera; Juniperus thurifera L; Leaves; Methanol; Methicillin; Plant extracts; Pseudomonas aeruginosa; Staphylococcus aureus infections; trapping test of 1
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2018.373-378

This work aimed to evaluate the biological activity of the leaf extracts of L., which is an Algerian endemic tree that belongs to the family of Cupressaceae. The plant leaves were extracted in solvents of increasing polarity to obtain different extracts such as methanol, petroleum ether, chloroform, ethyl acetate, and aqueous extracts (MeE, PEE, ChlE, EtAE, and AqE). The antioxidant activity of four extracts (MeE, ChlE, EtAE, and AqE) was assessed by trapping test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The evaluation of antibacterial activity of MeE, ChlE, EtAE, and PEE was done using the disk diffusion method on solid agar. The three extracts of EtAE, AqE, and MeE showed high antiradical activity toward the DPPH radical (IC =29.348 µg/mL, 37.538 µg/mL, and 52.573 µg/mL, respectively), while the lowest radical scavenging activity was expressed by the ChlE (IC =70.096 µg/mL). These extracts were active only toward the Gram-positive bacteria ( ATCC and methicillin-resistant ) at different concentrations, and the highest activity was obtained with the ChlE with an inhibition diameter of 14 mm at the concentration of 1 g/mL. No inhibition was detected for all of these extracts against the Gram-negative tested strains ( ATCC, ATCC, and (extended spectrum β-lactamase). From this study, on the one hand, it was concluded that L. leaves extracts exhibited a very intense antioxidant potential toward the DPPH radical, and on the other hand, the antibacterial activity showed an action spectrum exclusively toward the Gram-positive bacteria.