Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis

Oral submucous fibrosis (OSF) is a precancerous lesion. Adipose-derived stem cell- (ADSC-) derived extracellular vesicles (EVs) (ADSC-EVs) regulate multiple oral diseases. Hence, this study explored the mechanism of ADSC-EVs in OSF. ADSCs were transduced with microRNA- (miR-) 375 mimic. ADSC-EVs and...

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Bibliographic Details
Published in:Stem cells international 2021, Vol.2021, p.1-15
Main Authors: Han, Bin, Zhang, Yanhui, Xiao, Yuxia, Shi, Bohong, Wu, Hong, Liu, Desheng
Format: Article
Language:English
Subjects:
Antibiotics
Apoptosis
BAX protein
Bcl-2 protein
Caspase-3
Cell culture
Cell migration
Cell proliferation
Collagen
Collagen (type I)
Collagen (type III)
Extracellular vesicles
Fibroblasts
Fibrosis
Forkhead protein
Gene expression
Medical research
Medicine, Experimental
MicroRNA
MicroRNAs
miRNA
Mucosa
Oral diseases
Pathology
Proteins
Ribonucleic acid
RNA
Stem cells
Vesicles
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Summary:Oral submucous fibrosis (OSF) is a precancerous lesion. Adipose-derived stem cell- (ADSC-) derived extracellular vesicles (EVs) (ADSC-EVs) regulate multiple oral diseases. Hence, this study explored the mechanism of ADSC-EVs in OSF. ADSCs were transduced with microRNA- (miR-) 375 mimic. ADSC-EVs and miR-375-overexpressed ADSC-EVs (EVs-miR-375) were extracted and identified. miR-375 expression in EVs and fibrotic buccal mucosal fibroblasts (fBMFs) was detected. EV uptake by fBMFs was observed. The targeted relationship between miR-375 and forkhead box protein F1 (FOXF1) was predicted and verified. After EVs-miR-375 treatment or FOXF1 overexpression, fBMF cell proliferation, migration, invasion, and apoptosis were evaluated, and levels of apoptosis-related proteins (cleaved-caspase-3, Bax, and Bcl-2) and fibrosis markers (α-SMA, collagen I, and collagen III) were detected. Functional rescue experiments were further performed to verify the role of the miR-375/FOXF1 axis in OSF. miR-375 was notably upregulated in EVs-miR-375 and EVs-miR-375-treated fBMFs (all P
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2021/9964159