Identification of Uric Acid Crystals Accumulation in Human and Animal Tissues Using Combined Morphological and Raman Spectroscopy Analysis

Gout is a metabolic condition, common to animals and humans, issuing from the excessive accumulation of end products of proteins degradation. In this study, histopathological and cytological examinations, combined with Raman spectroscopy, have been performed to investigate tissue samples from reptil...

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Bibliographic Details
Published in:Diagnostics (Basel) 2022-11, Vol.12 (11), p.2762
Main Authors: Soare, Teodoru, Iordache, Ana Maria, Nicolae, George, Iordache, Stefan-Marian, Baciu, Cosmin, Marinescu, Silviu, Rizac, Raluca Ioana, Militaru, Manuella
Format: Article
Language:English
Subjects:
Animals
Bone surgery
crystals
gout
Health aspects
human
Laboratories
Measurement
Microscopy
Polymorphism
R&D
Raman spectroscopy
reptiles
Research & development
Rheumatism
Uric acid
Veterinary medicine
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Summary:Gout is a metabolic condition, common to animals and humans, issuing from the excessive accumulation of end products of proteins degradation. In this study, histopathological and cytological examinations, combined with Raman spectroscopy, have been performed to investigate tissue samples from reptiles, chickens, and humans, presenting lesions produced by uric acid accumulation. As a result of classic processing and staining techniques commonly used in the anatomopathological diagnosis, uric acid crystals lose their structural characteristics, thus making difficult a precise diagnostic. Therefore, complementary diagnostic methods, such as Raman spectroscopy, are needed. This study compares from several perspectives the above mentioned diagnostic methods, concluding that Raman spectroscopy provides highlights in the diagnosis of gout in humans and animals, also adding useful information to differential diagnosis of lesions.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics12112762