Association of Omentin rs2274907 and FTO rs9939609 gene polymorphisms with insulin resistance in Iranian individuals with newly diagnosed type 2 diabetes

• Published in: Lipids in health and disease 2019-06, Vol.18 (1), p.142-142, Article 142
• Main Authors: Khoshi, Amirhosein, Bajestani, Mehdi Kaffash, Shakeri, Habibesadat, Goodarzi, Golnaz, Azizi, Fatemeh
• Format: Article
• Language: English
• Subjects: Adipose tissue; Adult; Aged; Aged, 80 and over; Alpha-Ketoglutarate-Dependent Dioxygenase FTO; Blood glucose; Blood Glucose - metabolism; Body Mass Index; Body weight; Cardiovascular disease; Case-Control Studies; Cholesterol; Cytokines - genetics; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - diagnosis; Diabetes Mellitus, Type 2 - genetics; Diabetes therapy; Diagnosis; DNA; Enzymes; Fasting; Fat mass-and obesity associated (FTO); Gene polymorphism; Genes; Genetic aspects; Genetic diversity; Genetic polymorphisms; Genetic Predisposition to Disease - genetics; Genetic research; Genomes; Genotyping; Glucose; GPI-Linked Proteins - genetics; Homeostasis; Humans; Hyperglycemia; Hypertension; Insulin; Insulin resistance; Insulin Resistance - genetics; Insulin Resistance - physiology; Iran; Laboratories; Lectins - genetics; Metabolism; Middle Aged; Obesity; Omentin; Overweight; Polymerase chain reaction; Polymorphism; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Population; Restriction fragment length polymorphism; Studies; Type 2 diabetes; Young Adult
• ISSN: 1476-511X; 1476-511X
• DOI: 10.1186/s12944-019-1085-5

Insulin resistance (IR) and fat accumulation in visceral adipose tissue are key players in developing type 2 diabetes (T2D). Several adipose tissue derived-gene polymorphisms are related to higher body mass index (BMI), insulin resistance and T2D. The association of omentin rs2274907 (Val109Asp) and fat-mass and obesity-associated (FTO) rs9939609 gene polymorphisms with overweight/obesity and T2D is controversial. The aim of this study was to determine the association between omentin Val109Asp and FTO rs9939609 polymorphisms and insulin resistance in newly-diagnosed T2D patients. The case-control study included 83 newly-diagnosed T2D patients and 85 healthy matched controls, aged 20-80 years. Fasting blood glucose and insulin levels were measured by the enzymatic method and enzyme-linked-immunosorbent assay, respectively. Insulin resistance was calculated using the homeostasis model assessment (HOMA) index. Genotyping was examined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There are significant differences between both omentin Val109Asp and FTO rs9939609 polymorphisms and studied individuals (P = 0.011 and P = 0.0001, respectively). Both genetic polymorphisms of omentin Val109Asp and FTO rs9939609 (T/A) are significantly related to higher HOMA index (P = 0.030 and P = 0.046, respectively). However, omentin Val109Asp polymorphism was only related to individuals who were overweight/obese. Additionally, both omentin Val109Asp and FTO rs9939609 polymorphisms were significantly positively correlated to familial history of diabetes (P = 0.046 and P = 0.024, respectively). Omentin V109D and FTO rs9939609 genetic variations may change insulin metabolism and have key roles in developing T2D through insulin resistance. Thus, the evaluation of these polymorphic regions may be helpful for predicting type 2 diabetes.