Molecular characterization of constitutive heterochromatin in three species of Trypoxylon (Hymenoptera: Crabronidae: Trypoxylini) by CMA3/DAPI staining

Previous cytogenetic analyses in Trypoxylon Latreille, 1796 have been basically restricted to C-banding. In the present study, base-specific CMA3 and DAPI fluorochrome staining were used to characterize the constitutive heterochromatin in three Trypoxylon species. The heterochromatin was GC-rich in...

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Bibliographic Details
Published in:Comparative cytogenetics 2011, Vol.5 (2), p.71-80
Main Authors: Menezes, Rodolpho Santos Telles, Carvalho, Antonio Freire, Silva, Janisete Gomes, Costa, Marco Antonio
Format: Article
Language:English
Subjects:
4',6-Diamidino-2-phenylindole
Banding
Chromosome number
Euchromatin
Heterochromatin
Karyotypes
Species
Staining
Trypoxylon
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Summary:Previous cytogenetic analyses in Trypoxylon Latreille, 1796 have been basically restricted to C-banding. In the present study, base-specific CMA3 and DAPI fluorochrome staining were used to characterize the constitutive heterochromatin in three Trypoxylon species. The heterochromatin was GC-rich in all the species studied; however, in Trypoxylon nitidum F. Smith, 1856the molecular composition of the heterochromatinwasdifferent among chromosome pairs. Conversely, the euchromatin was AT-rich in the three species. These results suggest high conservatism in the euchromatic regions as opposed to the heterochromatic regions that have a high rate of changes. In this study, we report the karyotype of Trypoxylon rugifrons F. Smith, 1873which has the lowest chromosome number in the genus and other characteristics of the likely ancestral Trypoxylon karyotype.
ISSN:1993-0771
1993-078X
DOI:10.3897/compcytogen.v5i2.961