Damaged muscle fibers might masquerade as hybrid fibers - a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies

We report that, labeling mouse muscle tissue, with mouse monoclonal antibodies specific to slow or fast myosin heavy chain (sMyHC and fMyHC, respectively), can lead to artefactual labeling of damaged muscle fibers, as hybrid fibers (sMyHC+ and fMyHC+).  We demonstrate that such erroneous immunopheno...

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Bibliographic Details
Published in:European journal of histochemistry 2018-07, Vol.62 (3)
Main Authors: Begam, Morium, Roche, Joseph A
Format: Article
Language:English
Subjects:
Affinity Labels
Animals
Antibodies, Monoclonal - chemistry
Brief Report
fiber type
hybrid muscle fibers
immunolabeling
Immunophenotyping
Labeling
Mice
Mice, Inbred C57BL
Monoclonal antibodies
muscle fiber damage
Muscle, Skeletal
Myosin
myosin heavy chains
Myosin Heavy Chains - chemistry
Quadriceps muscle
Skeletal muscle
Tibialis anterior muscle
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Summary:We report that, labeling mouse muscle tissue, with mouse monoclonal antibodies specific to slow or fast myosin heavy chain (sMyHC and fMyHC, respectively), can lead to artefactual labeling of damaged muscle fibers, as hybrid fibers (sMyHC+ and fMyHC+).  We demonstrate that such erroneous immunophenotyping of muscle may be avoided, by performing colabeling or serial-section-labeling, to identify damaged fibers. The quadriceps femoris muscle group (QF) in 7-month-old, male, C57BL/6J mice had: 1.21 ± 0.21%, 98.34 ± 1.06%, 0.07 ± 0.01%, and 0.53 ± 0.85% fibers, that were, sMyHC+, fMyHC+, hybrid, and damaged, respectively.  All fibers in the tibialis anterior muscle (TA) of 3-month-old, male, C57BL/6J mice were fMyHC+; and at 3 days after injurious eccentric contractions, there was no fiber-type shift, but ~ 18% fibers were damaged.
ISSN:1121-760X
2038-8306
DOI:10.4081/ejh.2018.2896