Antioxidative and Anti-Atopic Dermatitis Effects of Peptides Derived from Hydrolyzed Sebastes schlegelii Tail By-Products

• Published in: Marine drugs 2024-10, Vol.22 (10), p.479
• Main Authors: Lee, Sung-Gyu, Hwang, Jin-Woo, Kang, Hyun
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Atopic dermatitis; Biological products; By products; Byproducts; Chemical properties; Chromatography; Chronic illnesses; Collagen; Cytokines; Dermatitis; Dermatitis, Atopic - chemically induced; Dermatitis, Atopic - drug therapy; Dermatologic agents; Dermatology; Dialysis; Disease Models, Animal; Drug therapy; Erythema; Fatty acids; Fish; Fishery products; Fishes; Formulae, receipts, prescriptions; Health aspects; High performance liquid chromatography; HPLC; Hydrolysates; Hydrolysis; Inflammation; Lesions; Leukocytes (eosinophilic); Liquid chromatography; Male; Marine fishes; Mice; Morbidity; Peptides; Peptides - chemistry; Peptides - isolation & purification; Peptides - pharmacology; Pharmaceutical research; Pharmacology; Protein hydrolysates; Proteins; Pruritus; Scavenging; Scorpaenidae; Sebastes schlegelii; Sebastes schlegelii tail by-products; Skin; Skin diseases; Skin lesions; STAT3; Subtilisin; Tails
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md22100479

Atopic dermatitis (AD) is a chronic inflammatory skin disorder associated with significant morbidity, including pruritus, recurrent skin lesions, and immune dysregulation. This study aimed to investigate the antioxidative and anti-AD effects of peptides derived from hydrolyzed (Korea rockfish) tail by-products. Hydrolysates were prepared using various enzymes, including Alcalase, Flavourzyme, Neutrase, and Protamex. Among them, Protamex hydrolysates demonstrated the highest ABTS radical scavenging activity with an RC value of 69.69 ± 0.41 µg/mL. Peptides were further isolated from the Protamex hydrolysate using dialysis, fast protein liquid chromatography (FPLC), and high-performance liquid chromatography (HPLC). The most active peptide, STPO-B-II, exhibited a single peak and was identified as a sequence of Glu-Leu-Ala-Lys-Thr-Trp-His-Asp-Met-Lys, designated as MP003. In vivo experiments were conducted using a 2,4-dinitrochlorbenzene (DNCB)-induced AD model in NC/Nga mice. The isolated peptide, MP003, showed significantly reduced AD symptoms, including erythema, lichenification, and collagen deposition. Additionally, MP003 decreased epidermal and dermal thickness, eosinophil, and mast cell infiltration and downregulated the expression of pro-inflammatory cytokines IL-1β, IL-6, and IgE in serum and skin tissues. These findings suggest that peptides derived from tail by-products may serve as potential therapeutic agents for AD.