Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system

The RAN Guanine Nucleotide Release Factor (RANGRF) gene encodes the protein MOG1, which binds to Nav1.5 and facilitates its transport to the cell membrane. Nav1.5 mutations have been linked to various cardiac arrhythmias and cardiomyopathy. To investigate the role of RANGRF in this process, we utili...

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Bibliographic Details
Published in:Stem cell research 2023-09, Vol.71, p.103136-103136, Article 103136
Main Authors: Jiang, Meiling, Tang, Chengcheng, Luo, Xian, Zhou, Xiaoqing, Chen, Min, Chi, Yue, Lai, Liangxue, Zou, Qingjian
Format: Article
Language:English
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Summary:The RAN Guanine Nucleotide Release Factor (RANGRF) gene encodes the protein MOG1, which binds to Nav1.5 and facilitates its transport to the cell membrane. Nav1.5 mutations have been linked to various cardiac arrhythmias and cardiomyopathy. To investigate the role of RANGRF in this process, we utilized the CRISPR/Cas9 gene editing system to generate a homozygous RANGRF knockout hiPSC line. The availability of the cell line will prove to be an invaluable asset in the study of disease mechanisms and the testing of gene therapies for cardiomyopathy.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2023.103136