Differences in Antioxidant and Lipid Handling Protein Expression Influence How Cells Expressing Distinct Mutant TP53 Subtypes Maintain Iron Homeostasis

The tumor suppressor TP53 is the most commonly mutated gene in human cancers, and iron is necessary for cancer cell growth and proliferation, but there is a significant gap in knowledge for how the two cooperate to affect cellular physiology. Elucidating this role is complicated, however, because ea...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2022-06, Vol.11 (13), p.2064
Main Authors: Cardona, Cameron J., Hermann, Evan R., Kouplen, Kate N., Hartson, Steven D., Montgomery, McKale R.
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Cancer
Chelation
ferroptosis
Gene expression
Homeostasis
Humidity
Iron
iron metabolism
Labeling
Mass spectroscopy
mutant TP53
Mutants
Mutation
Mutation hot spots
p53 Protein
Peptides
Proteins
Proteomics
Tumor suppressor genes
Tumors
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Summary:The tumor suppressor TP53 is the most commonly mutated gene in human cancers, and iron is necessary for cancer cell growth and proliferation, but there is a significant gap in knowledge for how the two cooperate to affect cellular physiology. Elucidating this role is complicated, however, because each TP53 mutation subtype exhibits unique phenotypic responses to changes in iron availability. The goal of this work was to determine how cells expressing distinct TP53 mutation subtypes respond to iron restriction. Utilizing a reverse genetics approach, we generated eight isogenic cell lines that either lacked TP53 expression, expressed wild-type TP53, or expressed one of the six most common TP53 “hotspot” mutations. We then employed isobaric peptide labeling and mass spectrometry to quantitively measure changes in global protein expression, both in response to induction of mutant TP53 expression, and in response to iron chelation. Our findings indicate that mutant TP53-dependent sensitivities to iron restriction are not driven by differences in responsiveness to iron chelation, but more so by mutant TP53-dependent differences in cellular antioxidant and lipid handling protein expression. These findings reinforce the importance of distinguishing between TP53 mutation subtypes when investigating approaches to target mutant TP53. We also identify unique TP53-dependent perturbances in protein expression patterns that could be exploited to improve iron-targeted chemotherapeutic strategies.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells11132064