Screening Antioxidant Components in Different Parts of Dandelion Using Online Gradient Pressure Liquid Extraction Coupled with High-Performance Liquid Chromatography Antioxidant Analysis System and Molecular Simulations

Utilizing online gradient pressure liquid extraction (OGPLE) coupled with a high-performance liquid chromatography antioxidant analysis system, we examined the antioxidative active components present in both the aerial parts and roots of dandelion. By optimizing the chromatographic conditions, we id...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2024-05, Vol.29 (10), p.2315
Main Authors: Cao, Xia, Li, Gaoquan, Xie, Juying, Wu, Mengqi, Wang, Wenhao, Xiao, Li, Qian, Zhengming
Format: Article
Language:English
Subjects:
Acids
antioxidant
Antioxidants
Antioxidants - chemistry
China
Chromatography
Chromatography, High Pressure Liquid - methods
dandelion
Efficiency
Electric power systems
High performance liquid chromatography
Instrument industry
Iron compounds
Ligands
Mass spectrometry
Methods
Molecular Docking Simulation
Natural products
Nitric oxide
online gradient pressure liquid extraction
Optimization
Oxidases
Plant Components, Aerial - chemistry
Plant Extracts - chemistry
Plant Roots - chemistry
Proteins
Simulation methods
Solvents
Superoxide
Taraxacum - chemistry
Taraxacum mongolicum
United States
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Summary:Utilizing online gradient pressure liquid extraction (OGPLE) coupled with a high-performance liquid chromatography antioxidant analysis system, we examined the antioxidative active components present in both the aerial parts and roots of dandelion. By optimizing the chromatographic conditions, we identified the ferric reducing-antioxidant power system as the most suitable for online antioxidant reactions in dandelion. Compared to offline ultrasonic extraction, the OGPLE method demonstrated superior efficiency in extracting chemical components with varying polarities from the samples. Liquid chromatography-mass spectrometry revealed twelve compounds within the dandelion samples, with nine demonstrating considerable antioxidant efficacy. Of these, the aerial parts and roots of dandelion contained nine and four antioxidant constituents, respectively. Additionally, molecular docking studies were carried out to investigate the interaction between these nine antioxidants and four proteins associated with oxidative stress (glutathione peroxidase, inducible nitric oxide synthase, superoxide dismutase, and xanthine oxidase). The nine antioxidant compounds displayed notable binding affinities below -5.0 kcal/mol with the selected proteins, suggesting potential receptor-ligand interactions. These findings contribute to enhancing our understanding of dandelion and provide a comprehensive methodology for screening the natural antioxidant components from herbs.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules29102315