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Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution...
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| Published in: | STAR protocols 2023-12, Vol.4 (4), p.102679-102679, Article 102679 |
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| container_end_page | 102679 |
| container_issue | 4 |
| container_start_page | 102679 |
| container_title | STAR protocols |
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| creator | Song, Jinyuan Zhang, Junlei Ji, Yongtao Tang, Jianghui Sheng, Jianpeng Liang, Tingbo Bai, Xueli |
| description | Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.
For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1
[Display omitted]
•Isolation of high-viability single cells from human pancreatic cancer tissue•Enrich immune cells using Percoll•Purify immune cells to make them suitable for FACS and scRNA-seq•Identification of major immune cell populations in pancreatic cancer using FACS
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. |
| doi_str_mv | 10.1016/j.xpro.2023.102679 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_10933901f07147aa97f1f1abf4466aec</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S2666166723006469</els_id><doaj_id>oai_doaj_org_article_10933901f07147aa97f1f1abf4466aec</doaj_id><sourcerecordid>2885540408</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3819-10051a780be7df62f5d4e0b0188a4e0718f1953066f2cee319bafd4e6432e85d3</originalsourceid><addsrcrecordid>eNp9kc1u1TAQhSMEolXpC7BAXrK5F4-TOI7EBlX8VKoEC1hbE2dcHDlxsBPo5Q14axxSKlYsbI9GZ77xzCmK58CPwEG-Go53cwxHwUWZE0I27aPiXEgpDyBl8_if-Ky4TGngnIsaRAXqaXFWNi3wGuC8-PUphiWY4JkNkbkUPC5uumUpX56YIe8TszGM7Os64sRmnEykrDHM5JAiW1xKKyWGU58P-tPPrX7EYeON4zrtFDaHed3gYUps3fDM-vCDmdMSRlri6VnxxKJPdHn_XhRf3r39fPXhcPPx_fXVm5uDKRW0B-D549go3lHTWyls3VfEOw5KYQ4aUBbauuRSWmGISmg7tFkiq1KQqvvyorjeuX3AQc_RjRhPOqDTfxIh3mqMeT5PGnhbli0Hm7FVg9g2FixgZ6tKSiSTWS93VrbiW17CokeXtmlxorAmLZSq64pXXGWp2KUmhpQi2YfWwPXmqB705qjeHNW7o7noxT1_7UbqH0r--pcFr3cB5Y19dxR1Mo6yL72LZJY8kvsf_zcTf7Qt</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2885540408</pqid></control><display><type>article</type><title>Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry</title><source>ScienceDirect®</source><source>PubMed Central</source><creator>Song, Jinyuan ; Zhang, Junlei ; Ji, Yongtao ; Tang, Jianghui ; Sheng, Jianpeng ; Liang, Tingbo ; Bai, Xueli</creator><creatorcontrib>Song, Jinyuan ; Zhang, Junlei ; Ji, Yongtao ; Tang, Jianghui ; Sheng, Jianpeng ; Liang, Tingbo ; Bai, Xueli</creatorcontrib><description>Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.
For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1
[Display omitted]
•Isolation of high-viability single cells from human pancreatic cancer tissue•Enrich immune cells using Percoll•Purify immune cells to make them suitable for FACS and scRNA-seq•Identification of major immune cell populations in pancreatic cancer using FACS
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.</description><identifier>ISSN: 2666-1667</identifier><identifier>EISSN: 2666-1667</identifier><identifier>DOI: 10.1016/j.xpro.2023.102679</identifier><identifier>PMID: 37910511</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cancer ; Cell Biology ; Cell Isolation ; Clinical Protocol ; Flow Cytometry ; Health Sciences ; Humans ; Pancreatic Neoplasms - genetics ; Single Cell ; Staining and Labeling</subject><ispartof>STAR protocols, 2023-12, Vol.4 (4), p.102679-102679, Article 102679</ispartof><rights>2023 The Authors</rights><rights>Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3819-10051a780be7df62f5d4e0b0188a4e0718f1953066f2cee319bafd4e6432e85d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S2666166723006469$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37910511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Jinyuan</creatorcontrib><creatorcontrib>Zhang, Junlei</creatorcontrib><creatorcontrib>Ji, Yongtao</creatorcontrib><creatorcontrib>Tang, Jianghui</creatorcontrib><creatorcontrib>Sheng, Jianpeng</creatorcontrib><creatorcontrib>Liang, Tingbo</creatorcontrib><creatorcontrib>Bai, Xueli</creatorcontrib><title>Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry</title><title>STAR protocols</title><addtitle>STAR Protoc</addtitle><description>Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.
For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1
[Display omitted]
•Isolation of high-viability single cells from human pancreatic cancer tissue•Enrich immune cells using Percoll•Purify immune cells to make them suitable for FACS and scRNA-seq•Identification of major immune cell populations in pancreatic cancer using FACS
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.</description><subject>Cancer</subject><subject>Cell Biology</subject><subject>Cell Isolation</subject><subject>Clinical Protocol</subject><subject>Flow Cytometry</subject><subject>Health Sciences</subject><subject>Humans</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Single Cell</subject><subject>Staining and Labeling</subject><issn>2666-1667</issn><issn>2666-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kc1u1TAQhSMEolXpC7BAXrK5F4-TOI7EBlX8VKoEC1hbE2dcHDlxsBPo5Q14axxSKlYsbI9GZ77xzCmK58CPwEG-Go53cwxHwUWZE0I27aPiXEgpDyBl8_if-Ky4TGngnIsaRAXqaXFWNi3wGuC8-PUphiWY4JkNkbkUPC5uumUpX56YIe8TszGM7Os64sRmnEykrDHM5JAiW1xKKyWGU58P-tPPrX7EYeON4zrtFDaHed3gYUps3fDM-vCDmdMSRlri6VnxxKJPdHn_XhRf3r39fPXhcPPx_fXVm5uDKRW0B-D549go3lHTWyls3VfEOw5KYQ4aUBbauuRSWmGISmg7tFkiq1KQqvvyorjeuX3AQc_RjRhPOqDTfxIh3mqMeT5PGnhbli0Hm7FVg9g2FixgZ6tKSiSTWS93VrbiW17CokeXtmlxorAmLZSq64pXXGWp2KUmhpQi2YfWwPXmqB705qjeHNW7o7noxT1_7UbqH0r--pcFr3cB5Y19dxR1Mo6yL72LZJY8kvsf_zcTf7Qt</recordid><startdate>20231215</startdate><enddate>20231215</enddate><creator>Song, Jinyuan</creator><creator>Zhang, Junlei</creator><creator>Ji, Yongtao</creator><creator>Tang, Jianghui</creator><creator>Sheng, Jianpeng</creator><creator>Liang, Tingbo</creator><creator>Bai, Xueli</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20231215</creationdate><title>Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry</title><author>Song, Jinyuan ; Zhang, Junlei ; Ji, Yongtao ; Tang, Jianghui ; Sheng, Jianpeng ; Liang, Tingbo ; Bai, Xueli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3819-10051a780be7df62f5d4e0b0188a4e0718f1953066f2cee319bafd4e6432e85d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Cancer</topic><topic>Cell Biology</topic><topic>Cell Isolation</topic><topic>Clinical Protocol</topic><topic>Flow Cytometry</topic><topic>Health Sciences</topic><topic>Humans</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Single Cell</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Jinyuan</creatorcontrib><creatorcontrib>Zhang, Junlei</creatorcontrib><creatorcontrib>Ji, Yongtao</creatorcontrib><creatorcontrib>Tang, Jianghui</creatorcontrib><creatorcontrib>Sheng, Jianpeng</creatorcontrib><creatorcontrib>Liang, Tingbo</creatorcontrib><creatorcontrib>Bai, Xueli</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>STAR protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Jinyuan</au><au>Zhang, Junlei</au><au>Ji, Yongtao</au><au>Tang, Jianghui</au><au>Sheng, Jianpeng</au><au>Liang, Tingbo</au><au>Bai, Xueli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry</atitle><jtitle>STAR protocols</jtitle><addtitle>STAR Protoc</addtitle><date>2023-12-15</date><risdate>2023</risdate><volume>4</volume><issue>4</issue><spage>102679</spage><epage>102679</epage><pages>102679-102679</pages><artnum>102679</artnum><issn>2666-1667</issn><eissn>2666-1667</eissn><abstract>Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.
For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).1
[Display omitted]
•Isolation of high-viability single cells from human pancreatic cancer tissue•Enrich immune cells using Percoll•Purify immune cells to make them suitable for FACS and scRNA-seq•Identification of major immune cell populations in pancreatic cancer using FACS
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>37910511</pmid><doi>10.1016/j.xpro.2023.102679</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | STAR protocols, 2023-12, Vol.4 (4), p.102679-102679, Article 102679 |
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| language | eng |
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| source | ScienceDirect®; PubMed Central |
| subjects | Cancer Cell Biology Cell Isolation Clinical Protocol Flow Cytometry Health Sciences Humans Pancreatic Neoplasms - genetics Single Cell Staining and Labeling |
| title | Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry |
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