Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals

In this study, new classes of tandemly repeated DNA sequences in the centromeric regions of three farm animal species are reported. Pericentromeric regions of bovine chromosome X, porcine chromosome 14 and equine chromosome 1 were microdissected by laser and amplified by DOP-PCR. Painting probes sho...

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Bibliographic Details
Published in:Veterinární medicína 2006-05, Vol.51 (5), p.224-231
Main Authors: Pauciullo, A.(University of Naples Federico II (Italy). Dept. of Animal Science and Food Inspection), Kubickova, S.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)), Cernohorska, H.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)), Petrova, K.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)), Di Berardino, D.(University of Naples Federico II (Italy). Dept. of Animal Science and Food Inspection), Ramunno, L.(University of Naples Federico II (Italy). Dept. of Animal Science and Food Inspection), Rubes, J.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) E-mail:rubes@vri.cz
Format: Article
Language:English
Subjects:
ADN
Animal species
bos taurus
BOVIN
CABALLOS
CATTLE
Centromeres
CERDO
CHEVAL
CHROMOSOME
Chromosome 1
Chromosome 14
CHROMOSOMES
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning
CROMOSOMAS
Deoxyribonucleic acid
DNA
DNA probes
equus caballus
fish
GANADO BOVINO
Gene sequencing
HIBRIDACION MOLECULAR
Homology
horse
HORSES
HYBRIDATION MOLECULAIRE
Hybridization
Localization
MOLECULAR CLONING
MOLECULAR HYBRIDIZATION
NUCLEOTIDE SEQUENCE
PCR
pig
PORCIN
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Species
sus scrofa
SWINE
tandem repeats
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Summary:In this study, new classes of tandemly repeated DNA sequences in the centromeric regions of three farm animal species are reported. Pericentromeric regions of bovine chromosome X, porcine chromosome 14 and equine chromosome 1 were microdissected by laser and amplified by DOP-PCR. Painting probes showed strong hybridization signals on their corresponding centromeres. Specific DNA fragments were isolated by cloning and sequenced. For each species, high homology level was found among the sequences. The presence of specific repeats within each sequence allows us to consider them as monomeric units or parts of longer tandemly repeated monomeric units that generate a specific higher-order repeat. In this respect, the isolation of new centromeric sequences enriches the genetic map of these three species and represents a useful tool for FISH analysis.
ISSN:0375-8427
1805-9392
DOI:10.17221/5541-VETMED