Detection of Anti-Neospora caninum IgG in Blood Serum and Colostrum Samples in Naturally Infected Sheep and in Their Newborn Offspring

The aim was to detect correlations of IgG antibodies against N. caninum in serum and colostrum samples from ewes, through the IFAT, and to evaluate the presence of this immunoglobulin in the serum of newborn lambs after colostrum ingestion. Blood samples from 162 ewes that did not show any disease i...

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Bibliographic Details
Published in:Pathogens (Basel) 2022-10, Vol.11 (11), p.1263
Main Authors: Bezerra, Roberto Alves, Lima, Brendo Andrade, Alvares, Felipe Boniedj Ventura, Rossi, Gabriel Augusto Marques, Braga, Fabio Ribeiro, de Melo, Renata Pimentel Bandeira, Mota, Rinaldo Aparecido, Vilela, Vinícius Longo Ribeiro, Feitosa, Thais Ferreira
Format: Article
Language:English
Subjects:
Abortion
Agreements
Animals
Antibodies
Births
Blood
Colostrum
Correlation
Cysts
Farms
Immunoglobulin G
Infections
Ingestion
Kappa coefficient
Lamb
Neonates
neosporosis
Offspring
ovine
Pregnancy
Serological tests
Serology
Sheep
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Summary:The aim was to detect correlations of IgG antibodies against N. caninum in serum and colostrum samples from ewes, through the IFAT, and to evaluate the presence of this immunoglobulin in the serum of newborn lambs after colostrum ingestion. Blood samples from 162 ewes that did not show any disease in the general physical examination and from their newborn lambs, not more than five days postpartum, along with 162 colostrum samples and 182 blood samples from the neonates, were analyzed. In total, 27.8% (45/162) of the mothers were positive for anti-N. caninum IgG, among which antibodies were detected in the colostrum in 46.7% (21/45). All the ewes with positive colostrum had reactive offspring. The kappa agreement for the correlation between the serological tests on the ewes and the colostrum results was 0.558. This correlation increased as the antibody titers of the mothers increased, and reached 1.000 from the titer of 1:400 from the mothers. Comparison of the antibody detection results between the offspring’s serum and colostrum showed a kappa agreement of 1.000. In conclusion, there was a good agreement regarding the detection of anti-N. caninum IgG between the colostrum samples and the lambs’ serum; the use of colostrum forms a noninvasive alternative for diagnosing N. caninum in sheep herds.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens11111263