Enzymatic Crosslinked Hydrogels of Gelatin and Poly (Vinyl Alcohol) Loaded with Probiotic Bacteria as Oral Delivery System

Probiotic bacteria are widely used to prepare pharmaceutical products and functional foods because they promote and sustain health. Nonetheless, probiotic viability is prone to decrease under gastrointestinal conditions. In this investigation, Lactiplantibacillus plantarum spp. CM-CNRG TB98 was entr...

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Published in:Pharmaceutics 2022-12, Vol.14 (12), p.2759
Main Authors: Corona-Escalera, Aldo F, Tinajero-Díaz, Ernesto, García-Reyes, Rudy A, Luna-Bárcenas, Gabriel, Seyfoddin, Ali, Padilla-de la Rosa, José Daniel, González-Ávila, Marisela, García-Carvajal, Zaira Y
Format: Article
Language:English
Subjects:
Bacteria
Biocompatibility
crosslinking
Dosage and administration
enzymatic
Enzymes
gelatin
Gels (Pharmacy)
hydrogel
Hydrogels
Materials
microbial transglutaminase
Nitrogen
NMR
Nuclear magnetic resonance
poly (vinyl alcohol)
Polymer blends
Polyvinyl alcohol
Probiotics
Scanning electron microscopy
Spectrum analysis
Thermogravimetric analysis
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Summary:Probiotic bacteria are widely used to prepare pharmaceutical products and functional foods because they promote and sustain health. Nonetheless, probiotic viability is prone to decrease under gastrointestinal conditions. In this investigation, Lactiplantibacillus plantarum spp. CM-CNRG TB98 was entrapped in a gelatin−poly (vinyl alcohol) (Gel−PVA) hydrogel which was prepared by a “green” route using microbial transglutaminase (mTGase), which acts as a crosslinking agent. The hydrogel was fully characterized and its ability to entrap and protect L. plantarum from the lyophilization process and under simulated gastric and intestine conditions was explored. The Gel−PVA hydrogel showed a high probiotic loading efficiency (>90%) and survivability from the lyophilization process (91%) of the total bacteria entrapped. Under gastric conditions, no disintegration of the hydrogel was observed, keeping L. plantarum protected with a survival rate of >94%. While in the intestinal fluid the hydrogel is completely dissolved, helping to release probiotics. A Gel−PVA hydrogel is suitable for a probiotic oral administration system due to its physicochemical properties, lack of cytotoxicity, and the protection it offers L. plantarum under gastric conditions.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14122759